Interleukin-8 of Cynoglossus semilaevis is a chemoattractant with immunoregulatory property
文献类型：期刊论文
| 作者 | Sun, Jin-sheng2; Zhao, Lu1,2; Sun, Li1 |
| 刊名 | FISH & SHELLFISH IMMUNOLOGY
 |
| 出版日期 | 2011-06-01 |
| 卷号 | 30期号:6页码:1362-1367 |
| 关键词 | Interleukin-8 Cynoglossus Semilaevis Inflammation Immunoregulatory |
| ISSN号 | 1050-4648 |
| DOI | 10.1016/j.fsi.2011.03.023 |
| 文献子类 | Article |
| 英文摘要 | Interleukin-8 (IL-8), or CXCL8, is a member of the CXC chemokine family that in mammals is known to mediate inflammatory response. In this study, we identified and analyzed an IL-8 orthologue, CsIL8, from half-smooth tongue sole (Cynoglossus semilaevis). The deduced amino acid sequence of CsIL8 contains 100 residues and is closely related to the lineage 1 IL-8 of a number of fish species. In silico analysis identified in CsIL8 a CXC chemokine domain that contains four conserved cysteine residues, two of which form the CXC signature motif of CXC chemokines. Expression of CsIL8 as determined by quantitative real time RT-PCR was detected in a wide range of tissues under normal physiological conditions and was upregulated by bacterial challenge and by vaccination with a subunit vaccine. Purified recombinant CsIL8 (rCsIL8) induced migration of peripheral blood leukocytes and head kidney (HK) lymphocytes and stimulated the proliferation of these cells in a dose-dependent manner. When rCsIL8 was added to the cell culture of HK lymphocytes, it upregulated the expression of interleukin-1 beta and CsIL8 in a time-dependent fashion. These results indicate that CsIL8 is a biologically active CXC chemokine with immunoregulatory activity and that CsIL8 is involved in pathogen-induced inflammatory response. (C) 2011 Elsevier Ltd. All rights reserved.; Interleukin-8 (IL-8), or CXCL8, is a member of the CXC chemokine family that in mammals is known to mediate inflammatory response. In this study, we identified and analyzed an IL-8 orthologue, CsIL8, from half-smooth tongue sole (Cynoglossus semilaevis). The deduced amino acid sequence of CsIL8 contains 100 residues and is closely related to the lineage 1 IL-8 of a number of fish species. In silico analysis identified in CsIL8 a CXC chemokine domain that contains four conserved cysteine residues, two of which form the CXC signature motif of CXC chemokines. Expression of CsIL8 as determined by quantitative real time RT-PCR was detected in a wide range of tissues under normal physiological conditions and was upregulated by bacterial challenge and by vaccination with a subunit vaccine. Purified recombinant CsIL8 (rCsIL8) induced migration of peripheral blood leukocytes and head kidney (HK) lymphocytes and stimulated the proliferation of these cells in a dose-dependent manner. When rCsIL8 was added to the cell culture of HK lymphocytes, it upregulated the expression of interleukin-1 beta and CsIL8 in a time-dependent fashion. These results indicate that CsIL8 is a biologically active CXC chemokine with immunoregulatory activity and that CsIL8 is involved in pathogen-induced inflammatory response. (C) 2011 Elsevier Ltd. All rights reserved. |
| 学科主题 | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| URL标识 | 查看原文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000291838400018 |
| 公开日期 | 2012-07-03 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/11882]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Tianjin Normal Univ, Coll Life Sci, Tianjin 300387, Peoples R China |
| 推荐引用方式 GB/T 7714 | Sun, Jin-sheng,Zhao, Lu,Sun, Li. Interleukin-8 of Cynoglossus semilaevis is a chemoattractant with immunoregulatory property[J]. FISH & SHELLFISH IMMUNOLOGY,2011,30(6):1362-1367. |
| APA | Sun, Jin-sheng,Zhao, Lu,&Sun, Li.(2011).Interleukin-8 of Cynoglossus semilaevis is a chemoattractant with immunoregulatory property.FISH & SHELLFISH IMMUNOLOGY,30(6),1362-1367. |
| MLA | Sun, Jin-sheng,et al."Interleukin-8 of Cynoglossus semilaevis is a chemoattractant with immunoregulatory property".FISH & SHELLFISH IMMUNOLOGY 30.6(2011):1362-1367. |
入库方式： OAI收割
来源：海洋研究所
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