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Isolation and characterization of a virulent Vibrio sp bacterium from clams (Meretrix meretrix) with mass mortality

文献类型：期刊论文

作者Yue, Xin1,2; Liu, Baozhong1; Sun, Li1
刊名JOURNAL OF INVERTEBRATE PATHOLOGY
出版日期2011-02-01
卷号106期号:2页码:242-249
关键词Meretrix Meretrix Vibrio Mlsa Tem
ISSN号0022-2011
DOI10.1016/j.jip.2010.10.006
文献子类Article
英文摘要MM5 was a bacterial strain isolated from moribund clam (Meretrix meretrix) collected from a farm with mass mortality outbreak. Primary genotypic and phenotypic identification including 16S rDNA sequence analysis, multilocus sequence analysis (MLSA) of four housekeeping genes (gapA, ftsZ, mreB and topA) and biochemical tests suggested that strain MM5 was a Vibrio species closest to but different from Vibrio furnissii. Our previous study indicated that MM5 could induce a high mortality of M. meretrix (Yue et al., 2010). Quantitative challenge test was performed in this study to further evaluate the pathogenic potential of MM5, which showed that at 84 h post-inoculation, the cumulative mortalities of the MM5-injected group were significantly higher than those of control groups (P < 0.05). Cytopathological and histopathological features of the clam infected by MM5 were carried out by transmission electron microscopy (TEM) and Hematoxylin and Eosin (H&E) staining, respectively. Cytopathologically, foci of MM5 were found in hepatocytes of the clam infected by MM5. In addition, cytopathological lesion was detected in foot of infected clam. Histopathologically, MM5 was detected in different tissues of infected clam, including hepatopancreas, mantle and gill. Challenge test combined with pathological features indicated that MM5 was virulent to M. meretrix. (c) 2010 Elsevier Inc. All rights reserved.; MM5 was a bacterial strain isolated from moribund clam (Meretrix meretrix) collected from a farm with mass mortality outbreak. Primary genotypic and phenotypic identification including 16S rDNA sequence analysis, multilocus sequence analysis (MLSA) of four housekeeping genes (gapA, ftsZ, mreB and topA) and biochemical tests suggested that strain MM5 was a Vibrio species closest to but different from Vibrio furnissii. Our previous study indicated that MM5 could induce a high mortality of M. meretrix (Yue et al., 2010). Quantitative challenge test was performed in this study to further evaluate the pathogenic potential of MM5, which showed that at 84 h post-inoculation, the cumulative mortalities of the MM5-injected group were significantly higher than those of control groups (P < 0.05). Cytopathological and histopathological features of the clam infected by MM5 were carried out by transmission electron microscopy (TEM) and Hematoxylin and Eosin (H&E) staining, respectively. Cytopathologically, foci of MM5 were found in hepatocytes of the clam infected by MM5. In addition, cytopathological lesion was detected in foot of infected clam. Histopathologically, MM5 was detected in different tissues of infected clam, including hepatopancreas, mantle and gill. Challenge test combined with pathological features indicated that MM5 was virulent to M. meretrix. (c) 2010 Elsevier Inc. All rights reserved.
学科主题Zoology
URL标识查看原文
语种英语
WOS记录号WOS:000286701600012
公开日期2012-07-03
源URL[http://ir.qdio.ac.cn/handle/337002/11886]  
专题海洋研究所_实验海洋生物学重点实验室
作者单位1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
推荐引用方式
GB/T 7714		 Yue, Xin,Liu, Baozhong,Sun, Li. Isolation and characterization of a virulent Vibrio sp bacterium from clams (Meretrix meretrix) with mass mortality[J]. JOURNAL OF INVERTEBRATE PATHOLOGY,2011,106(2):242-249.
APA Yue, Xin,Liu, Baozhong,&Sun, Li.(2011).Isolation and characterization of a virulent Vibrio sp bacterium from clams (Meretrix meretrix) with mass mortality.JOURNAL OF INVERTEBRATE PATHOLOGY,106(2),242-249.
MLA Yue, Xin,et al."Isolation and characterization of a virulent Vibrio sp bacterium from clams (Meretrix meretrix) with mass mortality".JOURNAL OF INVERTEBRATE PATHOLOGY 106.2(2011):242-249.

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来源：海洋研究所

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