Purification of 6xHis-tagged phycobiliprotein using zinc-decorated silica-coated magnetic nanoparticles
文献类型：期刊论文
| 作者 | Chen, Yingjie1,2; Jiang, Peng1; Liu, Shaofang1,2; Zhao, Hui1,2; Cui, Yulin1,2; Qin, Song1 |
| 刊名 | JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
 |
| 出版日期 | 2011-04-15 |
| 卷号 | 879期号:13-14页码:993-997 |
| 关键词 | 6xhis Tag Phycobiliprotein Silica-coated Magnetic Nanoparticles (Simnps) Zn(Ii) Purification Adsorption Behavior |
| ISSN号 | 1570-0232 |
| DOI | 10.1016/j.jchromb.2011.03.014 |
| 文献子类 | Article |
| 英文摘要 | Zinc-decorated silica-coated magnetic nanoparticles (ZnSiMNPs) were prepared by adsorbing zinc onto colloidal silica. These nanoparticles were used for the rapid purification of 6xHis-tagged recombinant phycobiliprotein. The surface changes in the magnetic nanoparticles after zinc adsorption were characterized by transmission electron microscopy. The adsorption of the 6xHis-tagged phycobiliprotein onto ZnSiMNPs in 10 mM PBS at 25 degrees C was found to follow the Langmuir isotherm. ZnSiMNPs could be used to extract 6xHis-tagged phycobiliprotein from lysate to single-band purity, as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. No spectral variation was observed in the purified phycobiliprotein. Thus, ZnSiMNPs served as a useful tool for the magnetic separation and delivery of the 6xHis-tagged phycobiliprotein. (C) 2011 Elsevier B.V. All rights reserved.; Zinc-decorated silica-coated magnetic nanoparticles (ZnSiMNPs) were prepared by adsorbing zinc onto colloidal silica. These nanoparticles were used for the rapid purification of 6xHis-tagged recombinant phycobiliprotein. The surface changes in the magnetic nanoparticles after zinc adsorption were characterized by transmission electron microscopy. The adsorption of the 6xHis-tagged phycobiliprotein onto ZnSiMNPs in 10 mM PBS at 25 degrees C was found to follow the Langmuir isotherm. ZnSiMNPs could be used to extract 6xHis-tagged phycobiliprotein from lysate to single-band purity, as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. No spectral variation was observed in the purified phycobiliprotein. Thus, ZnSiMNPs served as a useful tool for the magnetic separation and delivery of the 6xHis-tagged phycobiliprotein. (C) 2011 Elsevier B.V. All rights reserved. |
| 学科主题 | Biochemistry & Molecular Biology ; Chemistry |
| URL标识 | 查看原文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000290005400025 |
| 公开日期 | 2012-07-03 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/11926]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, Yingjie,Jiang, Peng,Liu, Shaofang,et al. Purification of 6xHis-tagged phycobiliprotein using zinc-decorated silica-coated magnetic nanoparticles[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2011,879(13-14):993-997. |
| APA | Chen, Yingjie,Jiang, Peng,Liu, Shaofang,Zhao, Hui,Cui, Yulin,&Qin, Song.(2011).Purification of 6xHis-tagged phycobiliprotein using zinc-decorated silica-coated magnetic nanoparticles.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,879(13-14),993-997. |
| MLA | Chen, Yingjie,et al."Purification of 6xHis-tagged phycobiliprotein using zinc-decorated silica-coated magnetic nanoparticles".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 879.13-14(2011):993-997. |
入库方式： OAI收割
来源：海洋研究所
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