Role of MicroRNA-26b in Glioma Development and Its Mediated Regulation on EphA2
文献类型：期刊论文
| 作者 | Wu, Ning1,4; Zhao, Xiangzhong1; Liu, Ming1; Liu, Haizhou1; Yao, Weicheng2; Zhang, Yuyan1; Cao, Shousong3; Lin, Xiukun1 |
| 刊名 | PLOS ONE
 |
| 出版日期 | 2011-01-14 |
| 卷号 | 6期号:1页码:e16264 |
| ISSN号 | 1932-6203 |
| DOI | 10.1371/journal.pone.0016264 |
| 文献子类 | Article |
| 英文摘要 | Background: MicroRNAs (miRNAs) are short, non-coding RNAs that regulate the expression of multiple target genes. Deregulation of miRNAs is common in human tumorigenesis. Low level expression of miR-26b has been found in glioma cells. However, its underlying mechanism of action has not been determined.; Background: MicroRNAs (miRNAs) are short, non-coding RNAs that regulate the expression of multiple target genes. Deregulation of miRNAs is common in human tumorigenesis. Low level expression of miR-26b has been found in glioma cells. However, its underlying mechanism of action has not been determined. Methodology/Principal Findings: Real-time PCR was employed to measure the expression level of miR-26b in glioma patients and cells. The level of miR-26b was inversely correlated with the grade of glioma. Ectopic expression of miR-26b inhibited the proliferation, migration and invasion of human glioma cells. A binding site for miR-26b was identified in the 3'UTR of EphA2. Over-expression of miR-26b in glioma cells repressed the endogenous level of EphA2 protein. Vasculogenic mimicry (VM) experiments were performed to further confirm the effects of miR-26b on the regulation of EphA2, and the results showed that miR-26b inhibited the VM processes which regulated by EphA2. Significance: This study demonstrated that miR-26b may act as a tumor suppressor in glioma and it directly regulates EphA2 expression. EphA2 is a direct target of miR-26b, and the down-regulation of EphA2 mediated by miR-26b is dependent on the binding of miR-26b to a specific response element of microRNA in the 3'UTR region of EphA2 mRNA. |
| 学科主题 | Life Sciences & Biomedicine - Other Topics |
| URL标识 | 查看原文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000286516500042 |
| 公开日期 | 2012-07-03 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/11932]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao, Peoples R China 2.Qingdao Med Univ, Inst Brain, Affiliated Hosp, Qingdao, Peoples R China 3.Roswell Pk Canc Inst, Buffalo, NY 14263 USA 4.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wu, Ning,Zhao, Xiangzhong,Liu, Ming,et al. Role of MicroRNA-26b in Glioma Development and Its Mediated Regulation on EphA2[J]. PLOS ONE,2011,6(1):e16264. |
| APA | Wu, Ning.,Zhao, Xiangzhong.,Liu, Ming.,Liu, Haizhou.,Yao, Weicheng.,...&Lin, Xiukun.(2011).Role of MicroRNA-26b in Glioma Development and Its Mediated Regulation on EphA2.PLOS ONE,6(1),e16264. |
| MLA | Wu, Ning,et al."Role of MicroRNA-26b in Glioma Development and Its Mediated Regulation on EphA2".PLOS ONE 6.1(2011):e16264. |
入库方式： OAI收割
来源：海洋研究所
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