Enhanced poly(3-hydroxypropionate) production via beta-alanine pathway in recombinant Escherichia coli
文献类型：期刊论文
| 作者 | Lacmata, Stephen Tamekou1,2; Kuiate, Jules-Roger2; Ding, Yamei3; Xian, Mo1; Liu, Huizhou1; Boudjeko, Thaddee4; Feng, Xinjun1; Zhao, Guang1,5 |
| 刊名 | PLOS ONE
 |
| 出版日期 | 2017-03-02 |
| 卷号 | 12期号:3 |
| DOI | 10.1371/journal.pone.0173150 |
| 文献子类 | Article |
| 英文摘要 | Poly(3-hydroxypropionate) (P3HP) is a thermoplastic with great compostability and biocompatibility, and can be produced through several biosynthetic pathways, in which the glycerol pathway achieved the highest P3HP production. However, exogenous supply of vitamin B-12 was required to maintain the activity of glycerol dehydratase, resulting in high production cost. To avoid the addition of VB12, we have previously constructed a P3HP biosynthetic route with beta-alanine as intermediate, and the present study aimed to improve the P3HP production of this pathway. L-aspartate decarboxylase PanD was found to be the rate-limiting enzyme in the beta-alanine pathway firstly. To improve the pathway efficiency, PanD was screened from four different sources (Escherichia coli, Bacillus subtilis, Pseudomonas fluorescens, and Corynebacterium glutamicum). And PanD from C. glutamicum was found to have the highest activity, the P3HP production was improved in flask cultivation with this enzyme. To further improve the production, the host strain was screened and the culture condition was optimized. Under optimal conditions, production and content of P3HP reached to 10.2 g/L and 39.1% (wt/wt [cell dry weight]) in an aerobic fed-batch fermentation. To date, this is the highest P3HP production without VB12. |
| 语种 | 英语 |
| WOS记录号 | WOS:000396011300081 |
| 版本 | 出版稿 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/136371]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Key Lab Biobased Mat, Qingdao, Peoples R China 2.Univ Dschang, Fac Sci, Dept Biochem, Lab Microbiol & Antimicrobials Subst, Dschang, Cameroon 3.Chinese Acad Sci, Inst Oceanol, Qingdao, Peoples R China 4.Univ Yaounde, Lab Phytoptotect & Valorizat Vegetals Resources, Biotechnol Nkolbisson Ctr, Dept Biochem, Yaounde, Cameroon 5.Randian Technol Co Ltd, Tianjin, Peoples R China |
| 推荐引用方式 GB/T 7714 | Lacmata, Stephen Tamekou,Kuiate, Jules-Roger,Ding, Yamei,et al. Enhanced poly(3-hydroxypropionate) production via beta-alanine pathway in recombinant Escherichia coli[J]. PLOS ONE,2017,12(3). |
| APA | Lacmata, Stephen Tamekou.,Kuiate, Jules-Roger.,Ding, Yamei.,Xian, Mo.,Liu, Huizhou.,...&Zhao, Guang.(2017).Enhanced poly(3-hydroxypropionate) production via beta-alanine pathway in recombinant Escherichia coli.PLOS ONE,12(3). |
| MLA | Lacmata, Stephen Tamekou,et al."Enhanced poly(3-hydroxypropionate) production via beta-alanine pathway in recombinant Escherichia coli".PLOS ONE 12.3(2017). |
入库方式： OAI收割
来源：海洋研究所
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