Interaction between granulin A and enolase 1 attenuates the migration and invasion of human hepatoma cells
文献类型：期刊论文
| 作者 | Chen, Xiaoliang1; Xu, Huanli1; Wu, Ning2; Liu, Xiujun3; Qiao, Gan1; Su, Shuonan1; Tian, Ye1; Yuan, Ru1; Li, Cong1; Liu, Xiaohui1 |
| 刊名 | ONCOTARGET
 |
| 出版日期 | 2017-05-02 |
| 卷号 | 8期号:18页码:30305-30316 |
| 关键词 | Enolase 1 Granulin a Invasion Migration Protein-protein Interaction |
| DOI | 10.18632/oncotarget.16328 |
| 文献子类 | Article |
| 英文摘要 | Granulin A (GRN A), a peptide with a molecular 6 kDa, is derived from proteolysis of progranulin (PGRN). Previous study in our laboratory has shown that GRN A is able to inhibit cancer cell growth significantly. In the present study, we confirmed that GRN A can bind to alpha-enolase (ENO1) specifically as analyzed using Pull-down/MS approaches. The interaction of GRN A with ENO1 was further confirmed by Western blotting and Surface plasmon resonance (SPR) analysis. Treatment of human HepG-2 cells with GRN A inhibited cancer cell growth as well as migration and invasion of cancer cells as analyzed by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazoliumbromide (MTT) and Scratch wound healing assay as well as Transwell experiments. Additionally, GRN A treatment results in augmentation of glucose uptake in cancer cells. Further study reveals that higher expression of ENO1 reversed the inhibitory effects of GRN A on migration and invasion of HepG-2 cells. The increase of glucose uptake, as well as the expression of apoptosis-related genes, is also reversed in cells overexpressing ENO1. The study provides solid evidence that there is the interaction between GRN A and ENO1 and the interaction is responsible for the effects of GRN A on glucose uptake as well as cancer cell migration and invasion. |
| 语种 | 英语 |
| WOS记录号 | WOS:000400456200061 |
| 版本 | 出版稿 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/137063]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Capital Med Univ, Dept Pharmacol, Beijing 100069, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Qingdao 266003, Peoples R China 3.Chinese Acad Med Sci, Inst Med Biotechnol, Beijing 100050, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, Xiaoliang,Xu, Huanli,Wu, Ning,et al. Interaction between granulin A and enolase 1 attenuates the migration and invasion of human hepatoma cells[J]. ONCOTARGET,2017,8(18):30305-30316. |
| APA | Chen, Xiaoliang.,Xu, Huanli.,Wu, Ning.,Liu, Xiujun.,Qiao, Gan.,...&Lin, Xiukun.(2017).Interaction between granulin A and enolase 1 attenuates the migration and invasion of human hepatoma cells.ONCOTARGET,8(18),30305-30316. |
| MLA | Chen, Xiaoliang,et al."Interaction between granulin A and enolase 1 attenuates the migration and invasion of human hepatoma cells".ONCOTARGET 8.18(2017):30305-30316. |
入库方式： OAI收割
来源：海洋研究所
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