Significant association of cypl9a promoter methylation with environmental factors and gonadal differentiation in olive flounder Paralichthys olivaceus
文献类型：期刊论文
| 作者 | Fan, Zhaofei1,2,3 ; Zou, Yuxia1,2; Jiao, Shuang1,2; Tan, Xungang1,2; Wu, Zhihao1,2; Liang, Dongdong1,2,3; Zhang, Peijun1,2; You, Feng1,2
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| 刊名 | COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY
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| 出版日期 | 2017-06-01 |
| 卷号 | 208页码:70-79 |
| 关键词 | Olive Flounder Paralichthys Olivaceus Cypl9a And Its Regulators Gene Expression Dna Methylation Gonadal Differentiation |
| DOI | 10.1016/j.cbpa.2017.02.017 |
| 文献子类 | Article |
| 英文摘要 | The sex ratio of olive flounder Paralichtilys olivaceus is sensitive to temperature or exogenous hormone exposures in the period of gonadal differentiation. Among sex-related genes, cypl9a, encoding cytochrome P450 aromatase, exhibits significant sex-dimorphic expression pattern and plays an important role in fish gonadal differentiation and development. The present study investigated the expression levels and promoter methylation dynamics of cypl9a and its regulators (nr5a2 and nr0b1), and sex-steroid hormone levels during flounder gonadal differentiation under the treatments of high temperature and estradio1-17 beta (E2). The results showed that levels of flounder cypl9a expression and estradio1-17 beta were repressed by high temperature treatment during this period. The up-regulation of nr5a2 by E2 treatment may be related to the all-female formation, and up-regulation of nrObl by high temperature treatment may be associated with masculinization. Co-transfection assay indicated that nr5a2 and nrObl were antagonist regulators of cypl9a. Furthermore, cypl9a promoter exhibited significant demethylation phenomenon at early stage of ovarian differentiation. While, high temperature could repress the demethylation process, resulting in hypermethylation maintenance in cypl9a promoter. The hypermethylation promoter was able to suppress cypl9a expression by blocking the nr5a2-mediated transactivation activity in vitro. The DNA methylation of epigenetic modification in cypl9a promoter might be the vital way linking environmental factors and gonadal differentiation in flounder. (C) 2017 Published by Elsevier Inc. |
| 语种 | 英语 |
| WOS记录号 | WOS:000401877100008 |
| 版本 | 出版稿 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/137085]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China 3.Univ Chinese Acad Sci, Beijing 10049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Fan, Zhaofei,Zou, Yuxia,Jiao, Shuang,et al. Significant association of cypl9a promoter methylation with environmental factors and gonadal differentiation in olive flounder Paralichthys olivaceus[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY,2017,208:70-79. |
| APA | Fan, Zhaofei.,Zou, Yuxia.,Jiao, Shuang.,Tan, Xungang.,Wu, Zhihao.,...&You, Feng.(2017).Significant association of cypl9a promoter methylation with environmental factors and gonadal differentiation in olive flounder Paralichthys olivaceus.COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY,208,70-79. |
| MLA | Fan, Zhaofei,et al."Significant association of cypl9a promoter methylation with environmental factors and gonadal differentiation in olive flounder Paralichthys olivaceus".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY 208(2017):70-79. |
入库方式： OAI收割
来源：海洋研究所
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