Molecular characterization and expression profiles of four transformer-2 isoforms in the Chinese mitten crab Eriocheir sinensis
文献类型：期刊论文
| 作者 | Luo Danli1,4; Liu Yuan1; Hui Min1; Song Chengwen1,4 ; Liu Hourong1,4; Cui Zhaoxia1,2,3
|
| 刊名 | CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
 |
| 出版日期 | 2017-07-01 |
| 卷号 | 35期号:4页码:782-791 |
| 关键词 | Eriocheir Sinensis Transformer-2 Genes Embryonic Stage Larval Stage Sex-determination |
| DOI | 10.1007/s00343-017-6071-z |
| 文献子类 | Article |
| 英文摘要 | The transformer-2 (tra-2) gene plays a key role in the regulatory hierarchy of sexual differentiation in somatic tissues and in the germline of Drosophila melanogaster. In this study, sequences and expression profiles of tra-2 in the Chinese mitten crab Eriocheir sinensis were characterized. Four tra-2 isoforms, designated as Estra-2a, Estra-2b, Estra-2c, and Estra-2d, were isolated. They all contained an RNA-recognition motif (RRM) and a linker region, which shared high similarity with other reported tra-2s. Sequence analysis revealed that Estra-2a, Estra-2b and Estra-2c are encoded by the same genomic locus and are generated by alternative splicing of the pre-mRNA. Compared with the other three isoforms, Estra-2d lacks the RS2 domain. Quantitative real-time PCR showed that all four isoforms were highly expressed in the fertilized egg, and in the 2-4 cell and blastula stages compared with larval stages (P <= 0.01), suggesting their maternal origin in early embryonic developmental stages. Notably, Estra-2a was highly expressed in male somatic tissues, while Estra-2c was significantly highly expressed in the ovary. These results suggest that Estra-2c is involved in sexual differentiation of the Chinese mitten crab. Our findings provide basic information for further functional studies of the tra-2 gene/protein in this species. |
| 语种 | 英语 |
| WOS记录号 | WOS:000403701200008 |
| 版本 | 出版稿 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/137121]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China 3.Natl & Local Joint Engn Lab Ecol Mariculture, Qingdao 266071, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Luo Danli,Liu Yuan,Hui Min,et al. Molecular characterization and expression profiles of four transformer-2 isoforms in the Chinese mitten crab Eriocheir sinensis[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2017,35(4):782-791. |
| APA | Luo Danli,Liu Yuan,Hui Min,Song Chengwen,Liu Hourong,&Cui Zhaoxia.(2017).Molecular characterization and expression profiles of four transformer-2 isoforms in the Chinese mitten crab Eriocheir sinensis.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,35(4),782-791. |
| MLA | Luo Danli,et al."Molecular characterization and expression profiles of four transformer-2 isoforms in the Chinese mitten crab Eriocheir sinensis".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 35.4(2017):782-791. |
入库方式： OAI收割
来源：海洋研究所
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