Specific capture of phosphopeptides by Zr4+-modified monolithic capillary column
文献类型：期刊论文
| 作者 | Dong, Jing; Zhou, Houjiang; Wu, Renan; Ye, Mingliang; Zou, Hanfa |
| 刊名 | journal of separation science
 |
| 出版日期 | 2007-11-01 |
| 卷号 | 30期号:17页码:2917-2923 |
| 关键词 | monolithic capillary column phosphoproteome analysis zirconium phosphate |
| ISSN号 | 1615-9306 |
| 产权排序 | 1;1 |
| 通讯作者 | 邹汉法 |
| 英文摘要 | a method to prepare zirconium phosphate (zrp)-modified monolithic capillary column for highly specific capture of phosphopeptides is presented. in this method, the phosphate monolithic capillary column was prepared by direct copolymerization of the functional monomer containing phosphate group (ethylene glycol methacrylate phosphate) and cross-linker (bis-acrylamide) in a ternary porogenic solvent. copolymerization of cross-linker and functional monomer simplifies the procedure for the preparation of phosphate chromatographic media. after zr4+ was immobilized, the zrp-modified monolithic capillary column was evaluated by the analysis of standard phosphoproteins and the excellent selectivity of this approach was demonstrated by analyzing phosphopeptides in the digest mixture of p-casein and bsa with molar ratio of 1:200. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, analytical |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | phosphoproteome analysis ; affinity-chromatography ; mass-spectrometry ; liquid-chromatography ; polymer monoliths ; zirconium ; enrichment ; peptides ; electrochromatography ; separation |
| 收录类别 | SCI |
| 原文出处 | true |
| 语种 | 英语 |
| WOS记录号 | WOS:000251461500016 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/99355]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Dong, Jing,Zhou, Houjiang,Wu, Renan,et al. Specific capture of phosphopeptides by Zr4+-modified monolithic capillary column[J]. journal of separation science,2007,30(17):2917-2923. |
| APA | Dong, Jing,Zhou, Houjiang,Wu, Renan,Ye, Mingliang,&Zou, Hanfa.(2007).Specific capture of phosphopeptides by Zr4+-modified monolithic capillary column.journal of separation science,30(17),2917-2923. |
| MLA | Dong, Jing,et al."Specific capture of phosphopeptides by Zr4+-modified monolithic capillary column".journal of separation science 30.17(2007):2917-2923. |
入库方式： OAI收割
来源：大连化学物理研究所
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