High temperature causes masculinization of genetically female olive flounder (Paralichthys olivaceus) accompanied by primordial germ cell proliferation detention
文献类型：期刊论文
| 作者 | Wang, Xueying1,2 ; Liu, Qinghua1,2; Xiao, Yongshuang1,2; Yang, Yang1,2,3; Wang, Yanfeng1,2; Song, Zongcheng4; You, Feng1,2; An, Hao1,2,3; Li, Jun1,2
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| 刊名 | AQUACULTURE
 |
| 出版日期 | 2017-10-01 |
| 卷号 | 479页码:808-816 |
| 关键词 | Paralichthys Olivaceus High Temperature Germ Cell Sex Differentiation |
| DOI | 10.1016/j.aquaculture.2017.07.019 |
| 文献子类 | Article |
| 英文摘要 | Sex determination is not only resort to a single genetic cascade but also regulated along a continuum of environmental and heritable factors in many fish species. Temperature is one of the most important non-genetic sex differentiation elements. Previous studies regarding high temperature masculinization of flatfish typically focused on sex ratios and reproductive endocrinology, but little was known about germ cells during the process. In this study, we investigated the dynamic changes of germ cells in terms of morphology and number employing serial-sectioning and the molecular investigation of expression of dnd and amh genes by qRT-PCR for qualitative and quantitative analysis of germ cell proliferation during sexual differentiation. Moreover, the proliferation activity of primordial germ cells was investigated using PCNA (proliferating cell nuclear antigen) immunohistochemistry. |
| 语种 | 英语 |
| WOS记录号 | WOS:000408034700099 |
| 版本 | 出版稿 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/143135]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 海洋研究所_海洋生物技术研发中心 海洋研究所_胶州湾海洋生态系统国家野外研究站 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Weihai Shenghang Aquat Prod Sci & Technol Co Ltd, Weihai 264200, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Xueying,Liu, Qinghua,Xiao, Yongshuang,et al. High temperature causes masculinization of genetically female olive flounder (Paralichthys olivaceus) accompanied by primordial germ cell proliferation detention[J]. AQUACULTURE,2017,479:808-816. |
| APA | Wang, Xueying.,Liu, Qinghua.,Xiao, Yongshuang.,Yang, Yang.,Wang, Yanfeng.,...&Li, Jun.(2017).High temperature causes masculinization of genetically female olive flounder (Paralichthys olivaceus) accompanied by primordial germ cell proliferation detention.AQUACULTURE,479,808-816. |
| MLA | Wang, Xueying,et al."High temperature causes masculinization of genetically female olive flounder (Paralichthys olivaceus) accompanied by primordial germ cell proliferation detention".AQUACULTURE 479(2017):808-816. |
入库方式： OAI收割
来源：海洋研究所
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