Identification and Functional Characterization of the Glycogen Synthesis Related Gene Glycogenin in Pacific Oysters (Crassostrea gigas)
文献类型：期刊论文
| 作者 | Li, Busu1,2,3,4; Meng, Jie1,2,5 ; Li, Li1,2,5; Liu, Sheng1,2,3,4; Wang, Ting1,2,3,4; Zhang, Guofan1,2,4
|
| 刊名 | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
 |
| 出版日期 | 2017-09-06 |
| 卷号 | 65期号:35页码:7764-7773 |
| 关键词 | Crassostrea Gigas Glycogenin Glycogen Biosynthesis Glycogen Synthase Alternative Splicing |
| DOI | 10.1021/acs.jafc.7b02720 |
| 文献子类 | Article |
| 英文摘要 | High glycogen levels in the Pacific oyster (Crassostrea gigas) contribute to its flavor, quality, and hardiness. Glycogenin (CgGN) is the priming glucosyltransferase that initiates glycogen biosynthesis. We characterized the full sequence and function of C. gigas CgGN. Three CgGN isoforms (CgGN-alpha, beta, and gamma) containing alternative exon regions were isolated. CgGN expression varied seasonally in the adductor muscle and gonadal area and was the highest in the adductor muscle. Autoglycosylation of CgGN can interact with glycogen synthase (CgGS) to complete glycogen synthesis. Subcellular localization analysis showed that CgGN isoforms and CgGS were located in the cytoplasm. Additionally, a site-directed mutagenesis experiment revealed that the Tyr200Phe and Tyr202Phe mutations could affect CgGN autoglycosylation. This is the first study of glycogenin function in marine bivalves. These findings will improve our understanding of glycogen synthesis and accumulation mechanisms in mollusks. The data are potentially useful for breeding high-glycogen oysters. |
| 语种 | 英语 |
| WOS记录号 | WOS:000410255400020 |
| 版本 | 出版稿 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/143165]  |
| 专题 | 海洋研究所_海洋生物技术研发中心 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Natl & Local Joint Engn Lab Ecol Mariculture, Qingdao 266071, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266000, Peoples R China 5.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries & Aquaculture, Qingdao 266000, Shandong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Busu,Meng, Jie,Li, Li,et al. Identification and Functional Characterization of the Glycogen Synthesis Related Gene Glycogenin in Pacific Oysters (Crassostrea gigas)[J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,2017,65(35):7764-7773. |
| APA | Li, Busu,Meng, Jie,Li, Li,Liu, Sheng,Wang, Ting,&Zhang, Guofan.(2017).Identification and Functional Characterization of the Glycogen Synthesis Related Gene Glycogenin in Pacific Oysters (Crassostrea gigas).JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,65(35),7764-7773. |
| MLA | Li, Busu,et al."Identification and Functional Characterization of the Glycogen Synthesis Related Gene Glycogenin in Pacific Oysters (Crassostrea gigas)".JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 65.35(2017):7764-7773. |
入库方式： OAI收割
来源：海洋研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。