Integrating the Vibrio-resistance phenotype and gene expression data for discovery of markers used for resistance evaluation in the clam Meretrix petechialis
文献类型：期刊论文
| 作者 | Jiang, Fengjuan1,2; Wang, Hongxia1; Yue, Xin1; Zhang, Shujing1,2; Liu, Baozhong1,3
|
| 刊名 | AQUACULTURE
 |
| 出版日期 | 2018 |
| 卷号 | 482页码:130-136 |
| 关键词 | Meretrix Petechialis Vibrio Parahaemolyticus Resistance Gene Expression Level Molecular Markers |
| DOI | 10.1016/j.aquaculture.2017.09.033 |
| 文献子类 | Article |
| 英文摘要 | In this study, gene expression data were used to select resistance-associated markers that indicate resistance phenotypes in the clam Meretrix petechialis. A total of 1772 clams from 23 full-sibling families were subjected to a Vibrio challenge test, and the results showed significant variation in resistance to vibriosis among different families. Three of the most resistant families and three of the most susceptible families were selected for screening of resistance-associated markers. Then, among 22 genes, 12 demonstrated a significant change in expression, and their expression levels were further analysed by t-test and linear regression analyses, respectively, between resistant and susceptible families. Overall, the results showed that the basal expression levels of Big-Def, BIRC7, NFIL3, CTL9 and Bax were associated with Vibrio-resistant phenotypes and could be considered candidate resistance-associated markers. Further, two M. petechialis strains that differed in Vibrio resistance were used to examine the effectiveness of these five resistance-associated genes. The results further confirmed that the expression of BIRC7 and NFIL3 were associated with Vibrio-resistant phenotypes. This study integrated the Vibrio-resistance phenotype and gene expression data for resistance evaluation and provided potential markers for the genetic breeding of high Vibrio-resistance strains in the clam M. petechialis. |
| 语种 | 英语 |
| WOS记录号 | WOS:000413434400020 |
| 版本 | 出版稿 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/143229]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266000, Peoples R China |
| 推荐引用方式 GB/T 7714 | Jiang, Fengjuan,Wang, Hongxia,Yue, Xin,et al. Integrating the Vibrio-resistance phenotype and gene expression data for discovery of markers used for resistance evaluation in the clam Meretrix petechialis[J]. AQUACULTURE,2018,482:130-136. |
| APA | Jiang, Fengjuan,Wang, Hongxia,Yue, Xin,Zhang, Shujing,&Liu, Baozhong.(2018).Integrating the Vibrio-resistance phenotype and gene expression data for discovery of markers used for resistance evaluation in the clam Meretrix petechialis.AQUACULTURE,482,130-136. |
| MLA | Jiang, Fengjuan,et al."Integrating the Vibrio-resistance phenotype and gene expression data for discovery of markers used for resistance evaluation in the clam Meretrix petechialis".AQUACULTURE 482(2018):130-136. |
入库方式： OAI收割
来源：海洋研究所
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