Purification and biochemical characterization of soluble methane monooxygenase hydroxylase from Methylosinus trichosporium IMV 3011
文献类型：期刊论文
| 作者 | Hua Shaofeng; Li Shuben; Xin Jiayin; Niu Jianzhong; Xia Chungu; Tan Haidong; Tang Wei |
| 刊名 | bioscience biotechnology and biochemistry
 |
| 出版日期 | 2007 |
| 卷号 | 71期号:1页码:122-129 |
| 关键词 | characterization hydroxylase phylogenetic analysis purification methane monooxygenases |
| 产权排序 | 2;6 |
| 通讯作者 | li shuben |
| 英文摘要 | methane monooxygenase hydroxylase was purified by chromatography and characterized by electrophoresis and spectroscopy. the molecular mass of hydroxylase was 201.3 kda as determined by gel filtration, whereas the total molecular mass was 234 kda as judged by sds-page. structure study indicated that the enzyme is a homodimer structure, consisting of three subunits, designated alpha, beta, and gamma, with molecular masses of 58 kda, 36 kda, and 23 kda respectively. ief analysis indicated that the enzyme has a pi of 5.2. the uv-vis spectrum of hydroxylase revealed an absorption peak near 281 run and a weak shoulder peak around 395 nm-420 nm, and a fluorescence spectrum revealed an emission peak at 341.3 nm. circular dichroism measurement indicated that hydroxylase mainly consists of alpha-helical regions. finally, phylogenetic analysis indicated that this strain is very close to methylosinus trichosporium ob3b. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemistry & molecular biology ; biotechnology & applied microbiology ; chemistry, applied ; food science & technology |
| 研究领域[WOS] | biochemistry & molecular biology ; biotechnology & applied microbiology ; chemistry ; food science & technology |
| 关键词[WOS] | methylococcus-capsulatus-bath ; methanotrophic bacteria ; crystal-structure ; component ; oxidation ; trichloroethylene ; ob3b ; gyj3 |
| 收录类别 | SCI |
| 原文出处 | true |
| 语种 | 英语 |
| WOS记录号 | WOS:000243915600016 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/99387]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, State Key Lab Oxo Synthesis & Select Oxidat, Lanzhou Inst Chem Phys, Lanzhou 730000, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Hua Shaofeng,Li Shuben,Xin Jiayin,et al. Purification and biochemical characterization of soluble methane monooxygenase hydroxylase from Methylosinus trichosporium IMV 3011[J]. bioscience biotechnology and biochemistry,2007,71(1):122-129. |
| APA | Hua Shaofeng.,Li Shuben.,Xin Jiayin.,Niu Jianzhong.,Xia Chungu.,...&Tang Wei.(2007).Purification and biochemical characterization of soluble methane monooxygenase hydroxylase from Methylosinus trichosporium IMV 3011.bioscience biotechnology and biochemistry,71(1),122-129. |
| MLA | Hua Shaofeng,et al."Purification and biochemical characterization of soluble methane monooxygenase hydroxylase from Methylosinus trichosporium IMV 3011".bioscience biotechnology and biochemistry 71.1(2007):122-129. |
入库方式： OAI收割
来源：大连化学物理研究所
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