Three-dimensional fluorescence characteristics of dissolved organic matter produced by Prorocentrum donghaiense Lu
文献类型：期刊论文
| 作者 | Zhao Weihong1; Wang Jiangtao2; Chen Meimei1 |
| 刊名 | CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
 |
| 出版日期 | 2009-08-01 |
| 卷号 | 27期号:3页码:564-569 |
| 关键词 | Dissolved Organic Matter Fluorescence Excitation-emission Matrix Spectrum Prorocentrum Donghaiense Lu |
| ISSN号 | 0254-4059 |
| DOI | 10.1007/s00343-009-9141-z |
| 文献子类 | Article |
| 英文摘要 | Filtration and cross-flow ultrafiltration techniques were used to separate culture media of Prorocentrum donghaiense at the exponential growth, stationary and decline stages into < 0.45 mu m filtrate, 100 kDa-0.45 mu m, 10-100 kDa and 1-10 kDa retentate and < 1 kDa ultrafiltrate fractions. The fluorescence. properties of different molecular weights of dissolved organic matter (DOM) were measured by excitation-emission matrix spectra. Protein-like and humic-like fluorophores were observed in the DOM produced by P. donghaiense. The central positions of protein-like fluorophores showed a red shift with prolonged growth duration, shifting from tyrosine-like properties at the exponential growth stage to tryptophan-like properties at the stationary and decline stages. The excitation wavelengths of protein-like fluorophores exhibited some change in the exponential growth and stationary stages with increased molecular size, but showed little change in the decline stage. However, the emission wavelengths in the decline stage exhibited a blue shift. Very distinct C type and A type peaks in humic-like fluorophores were observed. With a prolonged culture time, the intensities of both of the peaks became strong and the excitation wavelengths of peak A showed a red shift, while the A:C ratios fell. More than 94% of fluorescent DOM was in the lower than 1 kDa molecular weight fraction.; Filtration and cross-flow ultrafiltration techniques were used to separate culture media of Prorocentrum donghaiense at the exponential growth, stationary and decline stages into < 0.45 mu m filtrate, 100 kDa-0.45 mu m, 10-100 kDa and 1-10 kDa retentate and < 1 kDa ultrafiltrate fractions. The fluorescence. properties of different molecular weights of dissolved organic matter (DOM) were measured by excitation-emission matrix spectra. Protein-like and humic-like fluorophores were observed in the DOM produced by P. donghaiense. The central positions of protein-like fluorophores showed a red shift with prolonged growth duration, shifting from tyrosine-like properties at the exponential growth stage to tryptophan-like properties at the stationary and decline stages. The excitation wavelengths of protein-like fluorophores exhibited some change in the exponential growth and stationary stages with increased molecular size, but showed little change in the decline stage. However, the emission wavelengths in the decline stage exhibited a blue shift. Very distinct C type and A type peaks in humic-like fluorophores were observed. With a prolonged culture time, the intensities of both of the peaks became strong and the excitation wavelengths of peak A showed a red shift, while the A:C ratios fell. More than 94% of fluorescent DOM was in the lower than 1 kDa molecular weight fraction. |
| 学科主题 | Limnology ; Oceanography |
| URL标识 | 查看原文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000270742000020 |
| 公开日期 | 2010-12-22 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/2677]  |
| 专题 | 海洋研究所_海洋生态与环境科学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Ecol & Environm Sci, Qingdao 266071, Peoples R China 2.Ocean Univ China, Minist Educ, Key Lab Marine Chem Theory & Technol, Qingdao 266100, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao Weihong,Wang Jiangtao,Chen Meimei. Three-dimensional fluorescence characteristics of dissolved organic matter produced by Prorocentrum donghaiense Lu[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2009,27(3):564-569. |
| APA | Zhao Weihong,Wang Jiangtao,&Chen Meimei.(2009).Three-dimensional fluorescence characteristics of dissolved organic matter produced by Prorocentrum donghaiense Lu.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,27(3),564-569. |
| MLA | Zhao Weihong,et al."Three-dimensional fluorescence characteristics of dissolved organic matter produced by Prorocentrum donghaiense Lu".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 27.3(2009):564-569. |
入库方式： OAI收割
来源：海洋研究所
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