Molecular sequencing and analysis of soluble methane monooxygenase gene clusters from methanotroph Methylomonas sp. GYJ3
文献类型：期刊论文
| 作者 | Hua, Shao-feng; Li, Shu-ben; Tan, Hai-dong |
| 刊名 | world journal of microbiology & biotechnology
 |
| 出版日期 | 2007-03-01 |
| 卷号 | 23期号:3页码:323-330 |
| 关键词 | electron microscopy phylogenetic analysis sequence analysis soluble methane monooxygenase 16S rDNA |
| 产权排序 | 2;3 |
| 通讯作者 | shu-ben li |
| 英文摘要 | a methanotroph methylomonas sp. gyj3 was isolated, whose smmo genes and 16s rdna were sequenced and analysed, demonstrating that the bacterium might be a type i methanotroph (gamma-proteobacteria) and was closer to methylomonas sp. kswiii/kspiii. this result was consistent with the result previously determined by biochemistry and morphological taxonomy. sequence comparison among six open reading frames and the deduced amino acid sequences of the smmo genes from six strains revealed that the strain gyj3 had highly conserved regions in mmox with other strains, amounting to 78-99% homology at protein level and 71-97% homology at dna level. highly conserved sequences lay in two iron-binding regions. furthermore, scanning electron microscopy of the strain gyj3 showed rod shapes with a slightly bent configuration on the even surfaces and with plump bodies. |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 类目[WOS] | biotechnology & applied microbiology |
| 研究领域[WOS] | biotechnology & applied microbiology |
| 关键词[WOS] | methylosinus-trichosporium ob3b ; methylococcus-capsulatus bath ; hydroxylase component ; crystal-structure ; protein-b ; trichloroethylene ; bacteria ; expression ; oxidation ; degradation |
| 收录类别 | SCI |
| 原文出处 | true |
| 语种 | 英语 |
| WOS记录号 | WOS:000244323200003 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/99389]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Lanzhou Inst Chem Phys, State Key Lab Oxo Synth & Select Oxidat, Lanzhou 730000, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Hua, Shao-feng,Li, Shu-ben,Tan, Hai-dong. Molecular sequencing and analysis of soluble methane monooxygenase gene clusters from methanotroph Methylomonas sp. GYJ3[J]. world journal of microbiology & biotechnology,2007,23(3):323-330. |
| APA | Hua, Shao-feng,Li, Shu-ben,&Tan, Hai-dong.(2007).Molecular sequencing and analysis of soluble methane monooxygenase gene clusters from methanotroph Methylomonas sp. GYJ3.world journal of microbiology & biotechnology,23(3),323-330. |
| MLA | Hua, Shao-feng,et al."Molecular sequencing and analysis of soluble methane monooxygenase gene clusters from methanotroph Methylomonas sp. GYJ3".world journal of microbiology & biotechnology 23.3(2007):323-330. |
入库方式： OAI收割
来源：大连化学物理研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。