Investigation of Extration Method for Paralytic Shellfish Poisoning Toxins in Shellfish
文献类型：期刊论文
| 作者 | Jiang Tao1,2,3 ; Jiang Tian-Jiu3,4
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| 刊名 | CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
 |
| 出版日期 | 2008-11-01 |
| 卷号 | 36期号:11页码:1460-1464 |
| 关键词 | Paralytic Shellfish Poisoning Toxins High Performance Liquid Chromatography Extraction |
| ISSN号 | 0253-3820 |
| 文献子类 | Article |
| 英文摘要 | Me optimal conditions were established for the extraction of paralytic shellfish poisoning toxins from gonad of Chlamys nobills using acetic acid and hydrochloric acid in the concentration range of 0.04-1.0 mol/L. A 10-g portion of gonad of Chlamys nobilis was extracted by boiling for 5 min with 1.0 mL acetic acid and hydrochloric acid in a 50-mL beaker. Meanwhile, a portion of gonad of Chlamys nobilis was extracted by sonication in the solution of 0.3 mol/L HAc + 0.2 mol/L HCl for a total period of 5-30 min. The raw extract was centrifuged at 3500 r/min for 5 min and the pH of supernatant was adjusted from 2.0 to 4.0 by 0.1 mol/L NaOH or 5 mol/L HCL After passing through a Millipore ultrafiltration membrane (10000 MW cut-off), ultrafiltrate was then analyzed by HPLC. The results showed that hydrochloric acid in the concentration range of 0.25-1.0 mol/L caused a significant decrease of N-sulfocarbarnoyl-11-hydroxysulfate toxin C1 (C1), C2 and gonyautoxin 5 (GTX5) and the concomitant increase of GTX2,3. However, the amount of the three unstable toxins did not show any change using the extraction with acetic acid. Under the same concentration of acetic acid (0.3 mol/L) and hydrochloric acid (0.2 mol/L), the amount of C1 in the ultrasonic extraction was obviously lower than the boiling one, while C2 showed slightly higher than the latter.; Me optimal conditions were established for the extraction of paralytic shellfish poisoning toxins from gonad of Chlamys nobills using acetic acid and hydrochloric acid in the concentration range of 0.04-1.0 mol/L. A 10-g portion of gonad of Chlamys nobilis was extracted by boiling for 5 min with 1.0 mL acetic acid and hydrochloric acid in a 50-mL beaker. Meanwhile, a portion of gonad of Chlamys nobilis was extracted by sonication in the solution of 0.3 mol/L HAc + 0.2 mol/L HCl for a total period of 5-30 min. The raw extract was centrifuged at 3500 r/min for 5 min and the pH of supernatant was adjusted from 2.0 to 4.0 by 0.1 mol/L NaOH or 5 mol/L HCL After passing through a Millipore ultrafiltration membrane (10000 MW cut-off), ultrafiltrate was then analyzed by HPLC. The results showed that hydrochloric acid in the concentration range of 0.25-1.0 mol/L caused a significant decrease of N-sulfocarbarnoyl-11-hydroxysulfate toxin C1 (C1), C2 and gonyautoxin 5 (GTX5) and the concomitant increase of GTX2,3. However, the amount of the three unstable toxins did not show any change using the extraction with acetic acid. Under the same concentration of acetic acid (0.3 mol/L) and hydrochloric acid (0.2 mol/L), the amount of C1 in the ultrasonic extraction was obviously lower than the boiling one, while C2 showed slightly higher than the latter. |
| 学科主题 | Chemistry, Analytical |
| 语种 | 英语 |
| WOS记录号 | WOS:000261465900002 |
| 公开日期 | 2010-12-24 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/5349]  |
| 专题 | 海洋研究所_海洋生态与环境科学重点实验室 海洋研究所_海洋地质与环境重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 3.Jinan Univ, Dept Environm Engn, Guangzhou 510632, Guangdong, Peoples R China 4.S China Normal Univ, Coll Life Sci & Technol, Guangzhou 510632, Guangdong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Jiang Tao,Jiang Tian-Jiu. Investigation of Extration Method for Paralytic Shellfish Poisoning Toxins in Shellfish[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2008,36(11):1460-1464. |
| APA | Jiang Tao,&Jiang Tian-Jiu.(2008).Investigation of Extration Method for Paralytic Shellfish Poisoning Toxins in Shellfish.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,36(11),1460-1464. |
| MLA | Jiang Tao,et al."Investigation of Extration Method for Paralytic Shellfish Poisoning Toxins in Shellfish".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 36.11(2008):1460-1464. |
入库方式： OAI收割
来源：海洋研究所
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