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Protein phosphatase inhibition assay for detection of diarrhetic shellfish poison in oyster

文献类型:期刊论文

作者Li, AF; Yu, RC; Li, J; Tang, XH; Wang, YF; Yan, T; Zhou, MJ
刊名CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
出版日期2006-03-01
卷号34期号:3页码:283-287
关键词Protein Phosphatase Diarrhetic Shellfish Poison Okadaic Acid Oyster
ISSN号0253-3820
文献子类Article
英文摘要A method based on protein phosphatase enzyme activity inhibition for the detection of diarrhetic shellfish poison (DSP) was used to analyze the DSP toxicity in three oyster samples. Based on the standard dose-effect curve developed with a series of okadaic acid (OA) standard solutions, the DSP toxicity of the three oyster samples collected were screened, and the results showed that there were no OA and dinophysis toxins ( DTXs) in the samples without hydrolization. However, the OA toxicity could be detected in two of the hydrolyzed samples, and the OA toxicity of the two samples were 1.81 and 1.21 mu g OA eq./kg oyster, respectively.; A method based on protein phosphatase enzyme activity inhibition for the detection of diarrhetic shellfish poison (DSP) was used to analyze the DSP toxicity in three oyster samples. Based on the standard dose-effect curve developed with a series of okadaic acid (OA) standard solutions, the DSP toxicity of the three oyster samples collected were screened, and the results showed that there were no OA and dinophysis toxins ( DTXs) in the samples without hydrolization. However, the OA toxicity could be detected in two of the hydrolyzed samples, and the OA toxicity of the two samples were 1.81 and 1.21 mu g OA eq./kg oyster, respectively.
学科主题Chemistry, Analytical
语种英语
WOS记录号WOS:000236528300001
公开日期2010-12-24
源URL[http://ir.qdio.ac.cn/handle/337002/5439]  
专题海洋研究所_海洋生态与环境科学重点实验室
海洋研究所_海洋生物技术研发中心
作者单位1.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
推荐引用方式
GB/T 7714
Li, AF,Yu, RC,Li, J,et al. Protein phosphatase inhibition assay for detection of diarrhetic shellfish poison in oyster[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2006,34(3):283-287.
APA Li, AF.,Yu, RC.,Li, J.,Tang, XH.,Wang, YF.,...&Zhou, MJ.(2006).Protein phosphatase inhibition assay for detection of diarrhetic shellfish poison in oyster.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,34(3),283-287.
MLA Li, AF,et al."Protein phosphatase inhibition assay for detection of diarrhetic shellfish poison in oyster".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 34.3(2006):283-287.

入库方式: OAI收割

来源:海洋研究所

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