Effect of alginic acid decomposing bacterium on the growth of Laminaria japonica (Phaeophyceae)
文献类型：期刊论文
| 作者 | Wang, Y ; Tang, XX; Yang, Z; Yu, ZM
|
| 刊名 | JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA
 |
| 出版日期 | 2006 |
| 卷号 | 18期号:3页码:543-551 |
| 关键词 | Laminaria Japonica Alginic Acid Decomposing Bacterial Infection Growth Mechanism |
| ISSN号 | 1001-0742 |
| 文献子类 | Article |
| 英文摘要 | We collected the diseased blades of Laminaria japonica from Yantai Sea Farm from October to December 2002, and the alginic acid decomposing bacterium on the diseased blade was isolated and purified, and was identified as Alterornonas espejiana. This bacterium was applied as the causative pathogen to infect the blades of L. japonica under laboratory conditions. The aim of the present study was to identify the effects of the bacterium on the growth of L. japonica, and to find the possibly effective mechanism. Results showed that: (1) The blades of L. japonica exhibited symptoms of lesion, bleaching and deterioration when infected by the bacterium, and their growth and photosynthesis were dramatically suppressed. At the same time, the reactive oxygen species (ROS) generation enhanced obviously, and the relative membrane permeability increased significantly. The contents of malonaldehyde (MDA) and free fatty acid in the microsomol membrane greatly elevated, but the phospholipid content decreased. Result suggested an obvious peroxidation and deesterrification in the blades of L. japonica when infected by the bacterium. (2) The simultaneous assay on the antioxidant enzyme activities demonstrated that superoxide dismutase (SOD) and catalase (CAT) increased greatly when infected by the bacterium, but glutathione peroxidase (Gpx) and ascorbate peroxidase (APX) did not exhibit active responses to the bacterium throughout the experiment. (3) The histomorphological observations gave a distinctive evidence of the severity of the lesions as well as the relative abundance in the bacterial population on the blades after infection. The bacterium firstly invaded into the endodermis of L. japonica and gathered around there, and then resulted in the membrane damage, cells corruption and ultimately, the death of L. japonica.; We collected the diseased blades of Laminaria japonica from Yantai Sea Farm from October to December 2002, and the alginic acid decomposing bacterium on the diseased blade was isolated and purified, and was identified as Alterornonas espejiana. This bacterium was applied as the causative pathogen to infect the blades of L. japonica under laboratory conditions. The aim of the present study was to identify the effects of the bacterium on the growth of L. japonica, and to find the possibly effective mechanism. Results showed that: (1) The blades of L. japonica exhibited symptoms of lesion, bleaching and deterioration when infected by the bacterium, and their growth and photosynthesis were dramatically suppressed. At the same time, the reactive oxygen species (ROS) generation enhanced obviously, and the relative membrane permeability increased significantly. The contents of malonaldehyde (MDA) and free fatty acid in the microsomol membrane greatly elevated, but the phospholipid content decreased. Result suggested an obvious peroxidation and deesterrification in the blades of L. japonica when infected by the bacterium. (2) The simultaneous assay on the antioxidant enzyme activities demonstrated that superoxide dismutase (SOD) and catalase (CAT) increased greatly when infected by the bacterium, but glutathione peroxidase (Gpx) and ascorbate peroxidase (APX) did not exhibit active responses to the bacterium throughout the experiment. (3) The histomorphological observations gave a distinctive evidence of the severity of the lesions as well as the relative abundance in the bacterial population on the blades after infection. The bacterium firstly invaded into the endodermis of L. japonica and gathered around there, and then resulted in the membrane damage, cells corruption and ultimately, the death of L. japonica. |
| 学科主题 | Environmental Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000237568200022 |
| 公开日期 | 2010-12-24 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/5467]  |
| 专题 | 海洋研究所_海洋生态与环境科学重点实验室 海洋研究所_海洋腐蚀与防护研究发展中心 |
| 作者单位 | 1.Ocean Univ China, Coll Marine Life Sci, Qingdao 266003, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Y,Tang, XX,Yang, Z,et al. Effect of alginic acid decomposing bacterium on the growth of Laminaria japonica (Phaeophyceae)[J]. JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA,2006,18(3):543-551. |
| APA | Wang, Y,Tang, XX,Yang, Z,&Yu, ZM.(2006).Effect of alginic acid decomposing bacterium on the growth of Laminaria japonica (Phaeophyceae).JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA,18(3),543-551. |
| MLA | Wang, Y,et al."Effect of alginic acid decomposing bacterium on the growth of Laminaria japonica (Phaeophyceae)".JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA 18.3(2006):543-551. |
入库方式： OAI收割
来源：海洋研究所
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