Determination of tetrodotoxin by liquid chromatography coupled with electrospray ion-trap mass spectrometry
文献类型：期刊论文
| 作者 | Li Ai-Feng; Yu Ren-Cheng; Zhou Ming-Jiang |
| 刊名 | CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
 |
| 出版日期 | 2007-03-01 |
| 卷号 | 35期号:3页码:397-400 |
| 关键词 | Liquid Chromatography With Electrospray Ion-tap Mass Spectrometry Tetrodotoxin Selected Ion Monitoring Selected Reaction Monitoring |
| ISSN号 | 0253-3820 |
| 文献子类 | Article |
| 英文摘要 | Two methods for tetrodotoxin analysis using liquid chromatography coupled with electrospray iontrap mass spectrometry (LC-ESI-MS) have been established with C,, reversed phase column and hydrophilic interaction liquid chromatography (HILIC) column, respectively. Sensitivity and reproducibility of the methods were compared. The method using C-18 column in selected ion monitoring (SIM) mode had a detection limit (S/N = 3) of 120 pg, and a good linearity of the calibration curve was obtained for tetrodotoxin (r = 0. 9992). High reproducibility of the method was observed, with a relative standard deviation (RSD) below 10%. The method using HILIC column in SIM mode and selected reaction monitoring (SRM) mode had detection limits (S/N = 3) of 15 and 3.75 pg, respectively. Good linearity of the calibration curves was obtained for tetrodotoxin (r = 0. 9996 and 0. 9998 in SIM and SRM mode, respectively). T he reproducibility was high in SIM mode but relatively poor in SRM mode. Based on the results, the method using HILIC column in SIM mode was suggested for the analysis of tetrodotoxin with LC-MS system.; Two methods for tetrodotoxin analysis using liquid chromatography coupled with electrospray iontrap mass spectrometry (LC-ESI-MS) have been established with C,, reversed phase column and hydrophilic interaction liquid chromatography (HILIC) column, respectively. Sensitivity and reproducibility of the methods were compared. The method using C-18 column in selected ion monitoring (SIM) mode had a detection limit (S/N = 3) of 120 pg, and a good linearity of the calibration curve was obtained for tetrodotoxin (r = 0. 9992). High reproducibility of the method was observed, with a relative standard deviation (RSD) below 10%. The method using HILIC column in SIM mode and selected reaction monitoring (SRM) mode had detection limits (S/N = 3) of 15 and 3.75 pg, respectively. Good linearity of the calibration curves was obtained for tetrodotoxin (r = 0. 9996 and 0. 9998 in SIM and SRM mode, respectively). T he reproducibility was high in SIM mode but relatively poor in SRM mode. Based on the results, the method using HILIC column in SIM mode was suggested for the analysis of tetrodotoxin with LC-MS system. |
| 语种 | 英语 |
| WOS记录号 | WOS:000245735200018 |
| 公开日期 | 2010-12-30 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/6261]  |
| 专题 | 海洋研究所_海洋生态与环境科学重点实验室 |
| 作者单位 | 1.Ocean Univ China, Key Lab Marine Environm Sci & Ecol, Qingdao 266100, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li Ai-Feng,Yu Ren-Cheng,Zhou Ming-Jiang. Determination of tetrodotoxin by liquid chromatography coupled with electrospray ion-trap mass spectrometry[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2007,35(3):397-400. |
| APA | Li Ai-Feng,Yu Ren-Cheng,&Zhou Ming-Jiang.(2007).Determination of tetrodotoxin by liquid chromatography coupled with electrospray ion-trap mass spectrometry.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,35(3),397-400. |
| MLA | Li Ai-Feng,et al."Determination of tetrodotoxin by liquid chromatography coupled with electrospray ion-trap mass spectrometry".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 35.3(2007):397-400. |
入库方式： OAI收割
来源：海洋研究所
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