Screening of phage displayed human liver cDNA library against dexamethasone
文献类型：期刊论文
| 作者 | Yu, Xiaoming1,2; Zhao, Peng1,2; Zhang, Weibing1; Zhang, Lihua1; Zhang, Yukui1 |
| 刊名 | journal of pharmaceutical and biomedical analysis
 |
| 出版日期 | 2007-12-21 |
| 卷号 | 45期号:5页码:701-705 |
| 关键词 | phage display cDNA library dexamethasone interaction |
| 产权排序 | 1;1 |
| 通讯作者 | 张丽华 |
| 英文摘要 | this paper described an attempt to establish a new method to screen the target biomolecule from phage displayed cdna library against small molecule drug insoluble in water. dexamethasone was selected as the model drug, and the screening was carried out in an eppendorf tube packed with the drug. the whole procedure was monitored by pcr with the enriched specific phage clone as the template. after four rounds screening, the pcr products of selected phages with the lengths of 400 and 600 bp were sequenced, and revealed identical sequences with cytochrome c oxidase subunit iii and albumin respectively by genbank searching. furthermore, frontal analysis-capillary electrophoresis (fa-ce) was performed to study the interaction between dexamethasone and albumin, and the binding constant was calculated to be 1.153 x 10(3), validating the weak specific interaction between the drug and the target protein. all these results demonstrated that with insoluble drug as the solid phase directly, the screening of target large molecule expressed in phage display cdna library was feasible, which might pave an easy way to screen the candidate drug targets. (c) 2007 elsevier b.v. all rights reserved. |
| WOS标题词 | science & technology ; physical sciences ; life sciences & biomedicine |
| 类目[WOS] | chemistry, analytical ; pharmacology & pharmacy |
| 研究领域[WOS] | chemistry ; pharmacology & pharmacy |
| 关键词[WOS] | capillary-electrophoresis ; protein interactions ; binding-protein ; expression ; identification ; selection ; cloning ; surface |
| 收录类别 | SCI |
| 原文出处 | true |
| 语种 | 英语 |
| WOS记录号 | WOS:000252013300001 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/99465]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatograph Res & Anal Ctr, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yu, Xiaoming,Zhao, Peng,Zhang, Weibing,et al. Screening of phage displayed human liver cDNA library against dexamethasone[J]. journal of pharmaceutical and biomedical analysis,2007,45(5):701-705. |
| APA | Yu, Xiaoming,Zhao, Peng,Zhang, Weibing,Zhang, Lihua,&Zhang, Yukui.(2007).Screening of phage displayed human liver cDNA library against dexamethasone.journal of pharmaceutical and biomedical analysis,45(5),701-705. |
| MLA | Yu, Xiaoming,et al."Screening of phage displayed human liver cDNA library against dexamethasone".journal of pharmaceutical and biomedical analysis 45.5(2007):701-705. |
入库方式： OAI收割
来源：大连化学物理研究所
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