Integrated multilayer microfluidic device with a nanoporous membrane interconnect for online coupling of solid-phase extraction to microchip electrophoresis
文献类型：期刊论文
| 作者 | Long, Zhicheng; Shen, Zheng; Wu, Dapeng; Qin, Jianhua; Lin, Bingcheng |
| 刊名 | lab on a chip
 |
| 出版日期 | 2007 |
| 卷号 | 7期号:12页码:1819-1824 |
| 产权排序 | 1;1 |
| 通讯作者 | 林炳承 ; 秦建华 |
| 英文摘要 | an integrated microfluidic device was developed for online coupling of solid-phase extraction to microchip electrophoresis ( chip spe-ce). with a nanoporous membrane sandwiched between two pdms substrates, spe preconcentration and electrophoretic separation can be carried out in upper and lower fluidic layers, separately and sequentially. during the spe process, the thin membrane can act as a fluid isolator to prevent intermixing between two fluidic channels. however, when a pulse voltage is applied, the membrane becomes a gateable interconnect so that a small plug of concentrated analytes can be online injected into the lower channel for subsequent separations. this multilayer design provides a universal solution to online spe-ce hyphenation. both electroosmotic flow and hydrodynamic pumps have been adopted for spe operation. spe was performed on a 2.5 mm long microcolumn, with two weirs on both sides to retain the c-18-coated silica beads. rhodamine 123 and fitc-labelled ephedrine were used to test the operational performance of the hyphenation system. high separation efficiency and thousand-fold signal enhancement were achieved. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, multidisciplinary ; nanoscience & nanotechnology |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry ; science & technology - other topics |
| 关键词[WOS] | polymerase-chain-reaction ; pressure injection ; sample preparation ; chip ; separation ; systems ; dna ; poly(dimethylsiloxane) ; preconcentration ; amplification |
| 收录类别 | SCI |
| 原文出处 | true |
| 语种 | 英语 |
| WOS记录号 | WOS:000251121000035 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/99497]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Long, Zhicheng,Shen, Zheng,Wu, Dapeng,et al. Integrated multilayer microfluidic device with a nanoporous membrane interconnect for online coupling of solid-phase extraction to microchip electrophoresis[J]. lab on a chip,2007,7(12):1819-1824. |
| APA | Long, Zhicheng,Shen, Zheng,Wu, Dapeng,Qin, Jianhua,&Lin, Bingcheng.(2007).Integrated multilayer microfluidic device with a nanoporous membrane interconnect for online coupling of solid-phase extraction to microchip electrophoresis.lab on a chip,7(12),1819-1824. |
| MLA | Long, Zhicheng,et al."Integrated multilayer microfluidic device with a nanoporous membrane interconnect for online coupling of solid-phase extraction to microchip electrophoresis".lab on a chip 7.12(2007):1819-1824. |
入库方式： OAI收割
来源：大连化学物理研究所
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