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Cloning and nucleotide sequence of D-hydantoinase gene of marine polyphosphate-accumulating bacterium, Halomonas sp YSR-3

文献类型：期刊论文

作者Ren Shiying2; Li Xiangqian2; Jia Jianbo2; Liu Fei2; Xiao Tian1
刊名CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
出版日期2011-09-01
卷号29期号:5页码:917-921
关键词Halomonas Sp Ysr-3 D-hydantoinase Polyphosphate-accumulating Bacterium
ISSN号0254-4059
DOI10.1007/s00343-011-0071-1
文献子类Article
英文摘要Hydantoinase is involved in the production of optically pure amino acids from racemic 5-mono-substituted hydantoins. We measured the D-hydantoinase activity in marine Halomonas sp. YSR-3 and amplified the D-hydantoinase gene by PCR. The gene was inserted into vector pGM-T and transformed into E. coli TOP10. The positive transformants with the D-hydantoinase gene were sequenced. The sequenced fragment comprises 1 510 base pairs. The D-hydantoinase gene from YSR-3 is 77% similar to that from Pseudomonas entomophila L4 by searching against the NCBI databse. The protein product of the YSR-3 D-hydantoinase gene is 75%, 73%, and 70% similar to those from Pseudomonas fluorescens Pf-5, Marinomonas sp. MED121, and Burkholderia vietnamiensis G4, respectively. The difference of the D-hydantoinase gene between marine Halomonas sp. YSR-3 and other terrestrial organisms is distinct.; Hydantoinase is involved in the production of optically pure amino acids from racemic 5-mono-substituted hydantoins. We measured the D-hydantoinase activity in marine Halomonas sp. YSR-3 and amplified the D-hydantoinase gene by PCR. The gene was inserted into vector pGM-T and transformed into E. coli TOP10. The positive transformants with the D-hydantoinase gene were sequenced. The sequenced fragment comprises 1 510 base pairs. The D-hydantoinase gene from YSR-3 is 77% similar to that from Pseudomonas entomophila L4 by searching against the NCBI databse. The protein product of the YSR-3 D-hydantoinase gene is 75%, 73%, and 70% similar to those from Pseudomonas fluorescens Pf-5, Marinomonas sp. MED121, and Burkholderia vietnamiensis G4, respectively. The difference of the D-hydantoinase gene between marine Halomonas sp. YSR-3 and other terrestrial organisms is distinct.
学科主题Marine & Freshwater Biology ; Oceanography
URL标识查看原文
语种英语
WOS记录号WOS:000293632500001
公开日期2012-07-03
源URL[http://ir.qdio.ac.cn/handle/337002/11650]  
专题海洋研究所_海洋生态与环境科学重点实验室
作者单位1.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China
2.Huaiyin Inst Technol, Sch Life Sci & Chem Engn, Huaian 223003, Peoples R China
推荐引用方式
GB/T 7714		 Ren Shiying,Li Xiangqian,Jia Jianbo,et al. Cloning and nucleotide sequence of D-hydantoinase gene of marine polyphosphate-accumulating bacterium, Halomonas sp YSR-3[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2011,29(5):917-921.
APA Ren Shiying,Li Xiangqian,Jia Jianbo,Liu Fei,&Xiao Tian.(2011).Cloning and nucleotide sequence of D-hydantoinase gene of marine polyphosphate-accumulating bacterium, Halomonas sp YSR-3.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,29(5),917-921.
MLA Ren Shiying,et al."Cloning and nucleotide sequence of D-hydantoinase gene of marine polyphosphate-accumulating bacterium, Halomonas sp YSR-3".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 29.5(2011):917-921.

入库方式： OAI收割

来源：海洋研究所

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