Recognition of oxytocin by capillary electrochromatography with monolithic tetrapeptide-imprinted polymer used as the stationary phase
文献类型：期刊论文
| 作者 | Zheng, Chao; Liu, Zhaosheng; Gao, Ruyu; Zhang, Lihua; Zhang, Yukui |
| 刊名 | analytical and bioanalytical chemistry
 |
| 出版日期 | 2007-07-01 |
| 卷号 | 388期号:5-6页码:1137-1145 |
| 关键词 | molecularly imprinted polymer capillary electrochromatography monolithic column epitope approach oxytocin |
| 产权排序 | 4;4 |
| 通讯作者 | 张玉奎 |
| 英文摘要 | using yplg (tyr-pro-leu-gly), a tetrapeptide, as the template, an imprinted monolithic column was prepared and applied to the selective recognition of oxytocin based on the epitope approach and capillary electrochromatography (cec). by optimizing the polymerization solution in terms of functional monomer, cross-linking reagent, porogen, and imprinted template via cec evaluations of synthesized columns, an imprinted monolith with good recognition capacity (the imprinting factors for yplg and oxytocin were 4.499 and 4.013, respectively) and high column efficiency (theoretical plates for yplg and oxytocin were 22,995 plates/m and 16,952 plates/m, respectively) was achieved. in addition, the effects of various experimental parameters on the recognition of oxytocin, including the organic modifier content, the buffer concentration, and the ph value, were studied systematically. furthermore, a mixture of oxytocin and other proteins was analyzed using this monolithic cec column, and oxytocin was eluted much more slowly than other large biomolecules, which demonstrated the high selective recognition ability of such an imprinted monolith for oxytocin with plg (pro-leu-gly) as the epitope. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | molecular recognition ; epitope approach ; peptides ; separation ; media ; receptors ; proteins ; column ; acid |
| 收录类别 | SCI |
| 原文出处 | true |
| 语种 | 英语 |
| WOS记录号 | WOS:000247998700019 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/99527]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatograph R & A Ctr, Dalian 116023, Peoples R China 2.Nankai Univ, Coll Chem, Tianjin 300071, Peoples R China 3.Tianjin Med Univ, Coll Pharm, Tianjin 300203, Peoples R China 4.Nankai Univ, Inst Element Organ Chem, State Key Lab Element Organ Chem, Tianjin 300071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zheng, Chao,Liu, Zhaosheng,Gao, Ruyu,et al. Recognition of oxytocin by capillary electrochromatography with monolithic tetrapeptide-imprinted polymer used as the stationary phase[J]. analytical and bioanalytical chemistry,2007,388(5-6):1137-1145. |
| APA | Zheng, Chao,Liu, Zhaosheng,Gao, Ruyu,Zhang, Lihua,&Zhang, Yukui.(2007).Recognition of oxytocin by capillary electrochromatography with monolithic tetrapeptide-imprinted polymer used as the stationary phase.analytical and bioanalytical chemistry,388(5-6),1137-1145. |
| MLA | Zheng, Chao,et al."Recognition of oxytocin by capillary electrochromatography with monolithic tetrapeptide-imprinted polymer used as the stationary phase".analytical and bioanalytical chemistry 388.5-6(2007):1137-1145. |
入库方式： OAI收割
来源：大连化学物理研究所
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