The diversity and structure of marine protists in the coastal waters of China revealed by morphological observation and 454 pyrosequencing
文献类型：期刊论文
| 作者 | Liu, Yun1,2; Song, Shuqun1,2; Chen, Tiantian1,2,3 ; Li, Caiwen1,2
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| 刊名 | ESTUARINE COASTAL AND SHELF SCIENCE
 |
| 出版日期 | 2017-04-05 |
| 卷号 | 189页码:143-155 |
| 关键词 | Protist Dinoflagellate Diatom 454-pyrosequencing Morphological Observation |
| DOI | 10.1016/j.ecss.2017.03.019 |
| 文献子类 | Article |
| 英文摘要 | Pyrosequencing of the 18S rRNA gene has been widely adopted to study the eukaryotic diversity in various types of environments, and has an advantage over traditional morphology methods in exploring unknown microbial communities. To comprehensively assess the diversity and community composition of marine protists in the coastal waters of China, we applied both morphological observations and high throughput sequencing of the V2 and V3 regions of 18S rDNA simultaneously to analyze samples collected from the surface layer of the Yellow and East China Seas. Dinoflagellates, diatoms and ciliates were the three dominant protistan groups as revealed by the two methods. Diatoms were the first dominant protistan group in the microscopic observations, with Skeletonema mainly distributed in the nearshore eutrophic waters and Chaetoceros in higher temperature and higher pH waters. The mixotrophic dinoflagellates, Gymnodinium and Gyrodinium, were more competitive in the oligotrophic waters. The pyrosequencing method revealed an extensive diversity of dinoflagellates. Chaetoceros was the only dominant diatom group in the pyrosequencing dataset. Gyrodinium represented the most abundant reads and dominated the offshore oligotrophic protistan community as they were in the microscopic observations. The dominance of parasitic dinoflagellates in the pyrosequencing dataset, which were overlooked in the morphological observations, indicates more attention should be paid to explore the potential role of this group. Both methods provide coherent clustering of samples. Nutrient levels, salinity and pH were the main factors influencing the distribution of protists. This study demonstrates that different primer pairs used in the pyrosequencing will indicate different protistan community structures. A suitable marker may reveal more comprehensive composition of protists and provide valuable information on environmental drivers. (C) 2017 Elsevier Ltd. All rights reserved. |
| 语种 | 英语 |
| WOS记录号 | WOS:000400718600013 |
| 版本 | 出版稿 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/137045]  |
| 专题 | 海洋研究所_海洋生态与环境科学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao 266237, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Yun,Song, Shuqun,Chen, Tiantian,et al. The diversity and structure of marine protists in the coastal waters of China revealed by morphological observation and 454 pyrosequencing[J]. ESTUARINE COASTAL AND SHELF SCIENCE,2017,189:143-155. |
| APA | Liu, Yun,Song, Shuqun,Chen, Tiantian,&Li, Caiwen.(2017).The diversity and structure of marine protists in the coastal waters of China revealed by morphological observation and 454 pyrosequencing.ESTUARINE COASTAL AND SHELF SCIENCE,189,143-155. |
| MLA | Liu, Yun,et al."The diversity and structure of marine protists in the coastal waters of China revealed by morphological observation and 454 pyrosequencing".ESTUARINE COASTAL AND SHELF SCIENCE 189(2017):143-155. |
入库方式： OAI收割
来源：海洋研究所
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