Selection of housekeeping genes as internal controls for quantitative RT-PCR analysis of the veined rapa whelk (Rapana venosa)
文献类型:期刊论文
作者 | Song, Hao1,2; Dang, Xin3; He, Yuan-qiu3; Zhang, Tao1,4![]() |
刊名 | PEERJ
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出版日期 | 2017-05-31 |
卷号 | 5 |
关键词 | Internal Control Real-time Pcr Tissue Development Rapana Venosa |
DOI | 10.7717/peerj.3398 |
文献子类 | Article |
英文摘要 | Background. The veined rapa whelk Rapana venosa is an important commercial shellfish in China and quantitative real-time PCR (qRT-PCR) has become the standard method to study gene expression in R. venosa. For accurate and reliable gene expression results, qRT-PCR assays require housekeeping genes as internal controls, which display highly uniform expression in different tissues or stages of development. However, to date no studies have validated housekeeping genes in R. venosa for use as internal controls for qRT-PCR. |
语种 | 英语 |
WOS记录号 | WOS:000402560400008 |
版本 | 出版稿 |
源URL | [http://ir.qdio.ac.cn/handle/337002/137105] ![]() |
专题 | 海洋研究所_海洋生态与环境科学重点实验室 |
作者单位 | 1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Ocean Univ China, Coll Fisheries, Qingdao, Peoples R China 4.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao, Peoples R China |
推荐引用方式 GB/T 7714 | Song, Hao,Dang, Xin,He, Yuan-qiu,et al. Selection of housekeeping genes as internal controls for quantitative RT-PCR analysis of the veined rapa whelk (Rapana venosa)[J]. PEERJ,2017,5. |
APA | Song, Hao,Dang, Xin,He, Yuan-qiu,Zhang, Tao,&Wang, Hai-yan.(2017).Selection of housekeeping genes as internal controls for quantitative RT-PCR analysis of the veined rapa whelk (Rapana venosa).PEERJ,5. |
MLA | Song, Hao,et al."Selection of housekeeping genes as internal controls for quantitative RT-PCR analysis of the veined rapa whelk (Rapana venosa)".PEERJ 5(2017). |
入库方式: OAI收割
来源:海洋研究所
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