Ecdysteroid-responsive genes: E75 and Retinoid X Receptor (RXR), essential molt-regulating genes in the shrimp, Fenneropenaeus chinensis
文献类型：学位论文
| 作者 | JOSEPRIYA THANKARAJ ALPHONSAL |
| 答辩日期 | 2009-01-05 |
| 文献子类 | 博士 |
| 授予单位 | 中国科学院海洋研究所 |
| 授予地点 | 海洋研究所 |
| 关键词 | 蜕皮激素响应基因 E75和retinoid X受体 蜕皮 中国明对虾 |
| 其他题名 | Ecdysteroid-responsive genes: E75 and Retinoid X Receptor (RXR), essential molt-regulating genes in the shrimp, Fenneropenaeus chinensis |
| 英文摘要 | The study presented in this thesis is an attempt to explore the potential involvement of two important molt-regulating genes: generally called ecdysone responsive genes, E75 and RXR in the molting process of Chinese shrimp, Fenneropenaeus chinensis. Full-length cDNAs encoding FcE75 and FcRXR were obtained using PCR technique. Genomic structure of FcRXR with 7 introns depicts the existence of several isoforms in shrimp and we identified two, namely RXR-1 and RXR-2. Quantitative Real time PCR confirmed the prominent expression of FcE75 and FcRXR mRNAs in the late pre-molt period (D3) of shrimp. Moreover, a marked tissue-specific difference in the expression of FcE75 and FcRXR transcripts were also seen which revealed that the expression of FcE75 could be regulated in a tissue-specific manner. Interestingly, the application of double strand RNA into the shrimp efficiently decreased the FcE75 and FcRXR expression levels. Observations suggested that FcE75 silencing in vivo completely arrested the molting process and caused death of the shrimp. Analysis of the animals which failed to molt showed defective epidermal retraction, poor development of setae and new cuticle. On the other hand, silencing of both FcE75 & FcRXR in turn leads to considerable reduction in the levels of chitinase and chitinase 1 gene. Further, FcE75 silenced samples revealed reduced level of FcRXR expression and vice versa. These results indicate that FcE75 & FcRXR exert a prominent function during the molting process. We conclude that the ecdysone responsive genes FcE75 and FcRXR are essential for proper molting and survival of shrimp. This is the first report elucidating the function of these genes in the molting of decapod crustaceans. |
| 语种 | 中文 |
| 公开日期 | 2010-11-04 |
| 页码 | 127 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/802]  |
| 专题 | 海洋研究所_海洋环流与波动重点实验室 |
| 推荐引用方式 GB/T 7714 | JOSEPRIYA THANKARAJ ALPHONSAL. Ecdysteroid-responsive genes: E75 and Retinoid X Receptor (RXR), essential molt-regulating genes in the shrimp, Fenneropenaeus chinensis[D]. 海洋研究所. 中国科学院海洋研究所. 2009. |
入库方式： OAI收割
来源：海洋研究所
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