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Extracellular hydrolytic enzyme screening of culturable heterotrophic bacteria from deep-sea sediments of the Southern Okinawa Trough

文献类型：期刊论文

作者Dang, Hongyue1,2; Zhu, Hu2; Wang, Jing2; Li, Tiegang1
刊名WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
出版日期2009
卷号25期号:1页码:71-79
关键词Heterotrophic Bacteria Extracellular Hydrolytic Enzyme Exoenzyme Cold-adaptivity Extremophile Deep-sea Sediment Southern Okinawa Trough West Pacific Ocean
ISSN号0959-3993
DOI10.1007/s11274-008-9865-5
其他题名mannual of IR1
文献子类Article
英文摘要The Southern Okinawa Trough is an area of focused sedimentation due to particulate matter export from the shelf of the East China Sea and the island of Taiwan. In order to understand the geomicrobiological characteristics of this unique sedimentary environment, bacterial cultivations were carried out for an 8.61 m CASQ core sediment sample. A total of 98 heterotrophic bacterial isolates were characterized based on 16S rRNA gene phylogenetic analysis. These isolates can be grouped into four bacterial divisions, including 13 genera and more than 20 species. Bacteria of the gamma-Proteobacteria lineage, especially those from the Halomonas ( 27 isolates) and Psychrobacter ( 20 isolates) groups, dominate in the culturable bacteria assemblage. They also have the broadest distribution along the depth of the sediment. More than 72.4% of the isolates showed extracellular hydrolytic enzyme activities, such as amylases, proteases, lipases and Dnases, and nearly 59.2% were cold-adapted exoenzyme-producers. Several Halomonas strains show almost all the tested hydrolases activities. The wide distribution of exoenzyme activities in the isolates may indicate their important ecological role of element biogeochemical cycling in the studied deep-sea sedimentary environment.; The Southern Okinawa Trough is an area of focused sedimentation due to particulate matter export from the shelf of the East China Sea and the island of Taiwan. In order to understand the geomicrobiological characteristics of this unique sedimentary environment, bacterial cultivations were carried out for an 8.61 m CASQ core sediment sample. A total of 98 heterotrophic bacterial isolates were characterized based on 16S rRNA gene phylogenetic analysis. These isolates can be grouped into four bacterial divisions, including 13 genera and more than 20 species. Bacteria of the gamma-Proteobacteria lineage, especially those from the Halomonas ( 27 isolates) and Psychrobacter ( 20 isolates) groups, dominate in the culturable bacteria assemblage. They also have the broadest distribution along the depth of the sediment. More than 72.4% of the isolates showed extracellular hydrolytic enzyme activities, such as amylases, proteases, lipases and Dnases, and nearly 59.2% were cold-adapted exoenzyme-producers. Several Halomonas strains show almost all the tested hydrolases activities. The wide distribution of exoenzyme activities in the isolates may indicate their important ecological role of element biogeochemical cycling in the studied deep-sea sedimentary environment.
学科主题Biotechnology & Applied Microbiology
语种英语
WOS记录号WOS:000261413100010
公开日期2010-12-22
源URL[http://ir.qdio.ac.cn/handle/337002/3659]  
专题海洋研究所_海洋地质与环境重点实验室
作者单位1.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Geol & Environm, Qingdao 266071, Peoples R China
2.China Univ Petr E China, Ctr Bioengn & Biotechnol, Qingdao 266555, Peoples R China
推荐引用方式
GB/T 7714		 Dang, Hongyue,Zhu, Hu,Wang, Jing,et al. Extracellular hydrolytic enzyme screening of culturable heterotrophic bacteria from deep-sea sediments of the Southern Okinawa Trough[J]. WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY,2009,25(1):71-79.
APA Dang, Hongyue,Zhu, Hu,Wang, Jing,&Li, Tiegang.(2009).Extracellular hydrolytic enzyme screening of culturable heterotrophic bacteria from deep-sea sediments of the Southern Okinawa Trough.WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY,25(1),71-79.
MLA Dang, Hongyue,et al."Extracellular hydrolytic enzyme screening of culturable heterotrophic bacteria from deep-sea sediments of the Southern Okinawa Trough".WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY 25.1(2009):71-79.

入库方式： OAI收割

来源：海洋研究所

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