Expression and localization of a novel phosducin-like protein from amphioxus Branchiostoma belcheri
文献类型：期刊论文
| 作者 | Saren Gaowa1,2; Zhao Yonggang3 |
| 刊名 | CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
 |
| 出版日期 | 2009-06-01 |
| 卷号 | 27期号:2页码:324-330 |
| 关键词 | Amphioxus B. B.lcheri Amphi-phlp Northern Blotting In Situ Hybridization Cellular Localization |
| ISSN号 | 0254-4059 |
| DOI | 10.1007/s00343-009-9120-4 |
| 文献子类 | Article |
| 英文摘要 | A full length amphioxus cDNA, encoding a novel phosducin-like protein (Amphi-PhLP), was identified for the first time from the gut cDNA library of Branchiostoma belcheri. It is comprised of 1 550 bp and an open reading frame (ORF) of 241 amino acids, with a predicted molecular mass of approximately 28 kDa. In situ hybridization histochemistry revealed a tissue-specific expression pattern of Amphi-PhLP with the high levels in the ovary, and at a lower level in the hind gut and testis, hepatic caecum, gill, endostyle, and epipharyngeal groove, while it was absent in the muscle, neural tube and notochord. In the Chinese Hamster Ovary (CHO) cells transfected with the expression plasmid pEGFP-N1/Amphi-PhLP, the fusion protein was targeted in the cytoplasm of CHO cells, suggesting that Amphi-PhLP is a cytosolic protein. This work may provide a framework for further understanding of the physiological function of Amphi-PhLP in B. belcheri.; A full length amphioxus cDNA, encoding a novel phosducin-like protein (Amphi-PhLP), was identified for the first time from the gut cDNA library of Branchiostoma belcheri. It is comprised of 1 550 bp and an open reading frame (ORF) of 241 amino acids, with a predicted molecular mass of approximately 28 kDa. In situ hybridization histochemistry revealed a tissue-specific expression pattern of Amphi-PhLP with the high levels in the ovary, and at a lower level in the hind gut and testis, hepatic caecum, gill, endostyle, and epipharyngeal groove, while it was absent in the muscle, neural tube and notochord. In the Chinese Hamster Ovary (CHO) cells transfected with the expression plasmid pEGFP-N1/Amphi-PhLP, the fusion protein was targeted in the cytoplasm of CHO cells, suggesting that Amphi-PhLP is a cytosolic protein. This work may provide a framework for further understanding of the physiological function of Amphi-PhLP in B. belcheri. |
| 学科主题 | Limnology ; Oceanography |
| 语种 | 英语 |
| WOS记录号 | WOS:000267970300017 |
| 公开日期 | 2010-12-22 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/3695]  |
| 专题 | 海洋研究所_海洋地质与环境重点实验室 |
| 作者单位 | 1.Ocean Univ China, Dept Marine Biol, Qingdao 266003, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Geol & Environm, Qingdao 266071, Peoples R China 3.China Anim Hlth & Epidemiol Ctr, Qingdao 266032, Peoples R China |
| 推荐引用方式 GB/T 7714 | Saren Gaowa,Zhao Yonggang. Expression and localization of a novel phosducin-like protein from amphioxus Branchiostoma belcheri[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2009,27(2):324-330. |
| APA | Saren Gaowa,&Zhao Yonggang.(2009).Expression and localization of a novel phosducin-like protein from amphioxus Branchiostoma belcheri.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,27(2),324-330. |
| MLA | Saren Gaowa,et al."Expression and localization of a novel phosducin-like protein from amphioxus Branchiostoma belcheri".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 27.2(2009):324-330. |
入库方式： OAI收割
来源：海洋研究所
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