中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
首页 机构 成果 学者
  
  1. CAS IR Grid
  2. 海洋研究所
  3. 中国科学院海洋研究所
  4. 海洋地质与环境重点实验室
Diversity of Both the Cultivable Protease-Producing Bacteria and Their Extracellular Proteases in the Sediments of the South China Sea

文献类型：期刊论文

作者Zhou, Ming-Yang1; Chen, Xiu-Lan1; Zhao, Hui-Lin1; Dang, Hong-Yue2; Luan, Xi-Wu3; Zhang, Xi-Ying1; He, Hai-Lun1; Zhou, Bai-Cheng1; Zhang, Yu-Zhong1
刊名MICROBIAL ECOLOGY
出版日期2009-10-01
卷号58期号:3页码:582-590
ISSN号0095-3628
关键词Deep-sea Subseafloor Sediments Seep Carbonates Sp Sm9913 Psychrotolerant Communities Strain Ocean
DOI10.1007/s00248-009-9506-z
文献子类Article
英文摘要Protease-producing bacteria are known to play an important role in degrading sedimentary particular organic nitrogen, and yet, their diversity and extracellular proteases remain largely unknown. In this paper, the diversity of the cultivable protease-producing bacteria and their extracellular proteases in the sediments of the South China Sea was investigated. The richness of the cultivable protease-producing bacteria reached 10(6) cells/g in all sediment samples. Analysis of the 16S rRNA gene sequences revealed that the predominant cultivated protease-producing bacteria are Gammaproteobacteria affiliated with the genera Pseudoalteromonas, Alteromonas, Marinobacter, Idiomarina, Halomonas, Vibrio, Shewanella, Pseudomonas, and Rheinheimera, with Alteromonas (34.6%) and Pseudoalteromonas (28.2%) as the predominant groups. Inhibitor analysis showed that nearly all the extracellular proteases from the bacteria are serine proteases or metalloproteases. Moreover, these proteases have different hydrolytic ability to different proteins, reflecting they may belong to different kinds of serine proteases or metalloproteases. To our knowledge, this study represents the first report of the diversity of bacterial proteases in deep-sea sediments.; Protease-producing bacteria are known to play an important role in degrading sedimentary particular organic nitrogen, and yet, their diversity and extracellular proteases remain largely unknown. In this paper, the diversity of the cultivable protease-producing bacteria and their extracellular proteases in the sediments of the South China Sea was investigated. The richness of the cultivable protease-producing bacteria reached 10(6) cells/g in all sediment samples. Analysis of the 16S rRNA gene sequences revealed that the predominant cultivated protease-producing bacteria are Gammaproteobacteria affiliated with the genera Pseudoalteromonas, Alteromonas, Marinobacter, Idiomarina, Halomonas, Vibrio, Shewanella, Pseudomonas, and Rheinheimera, with Alteromonas (34.6%) and Pseudoalteromonas (28.2%) as the predominant groups. Inhibitor analysis showed that nearly all the extracellular proteases from the bacteria are serine proteases or metalloproteases. Moreover, these proteases have different hydrolytic ability to different proteins, reflecting they may belong to different kinds of serine proteases or metalloproteases. To our knowledge, this study represents the first report of the diversity of bacterial proteases in deep-sea sediments.
学科主题Ecology ; Marine & Freshwater Biology ; Microbiology
语种英语
WOS记录号WOS:000269928300014
公开日期2010-12-22
源URL[http://ir.qdio.ac.cn/handle/337002/3735]  
专题海洋研究所_海洋地质与环境重点实验室
作者单位1.Shandong Univ, State Key Lab Microbial Technol, Marine Biotechnol Res Ctr, Jinan 250100, Peoples R China
2.China Univ Petr E China, Ctr Bioengn & Biotechnol, Qingdao 266555, Peoples R China
3.Chinese Acad Sci, Key Lab Marine Geol & Environm, Inst Oceanol, Qingdao 266071, Peoples R China
推荐引用方式
GB/T 7714		 Zhou, Ming-Yang,Chen, Xiu-Lan,Zhao, Hui-Lin,et al. Diversity of Both the Cultivable Protease-Producing Bacteria and Their Extracellular Proteases in the Sediments of the South China Sea[J]. MICROBIAL ECOLOGY,2009,58(3):582-590.
APA Zhou, Ming-Yang.,Chen, Xiu-Lan.,Zhao, Hui-Lin.,Dang, Hong-Yue.,Luan, Xi-Wu.,...&Zhang, Yu-Zhong.(2009).Diversity of Both the Cultivable Protease-Producing Bacteria and Their Extracellular Proteases in the Sediments of the South China Sea.MICROBIAL ECOLOGY,58(3),582-590.
MLA Zhou, Ming-Yang,et al."Diversity of Both the Cultivable Protease-Producing Bacteria and Their Extracellular Proteases in the Sediments of the South China Sea".MICROBIAL ECOLOGY 58.3(2009):582-590.

入库方式： OAI收割

来源：海洋研究所

浏览0
下载0
收藏0
其他版本

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。