Microfluidic chip-based cell electrophoresis with multipoint laser-induced fluorescence detection system
文献类型：期刊论文
| 作者 | Yu, Linfen1; Shen, Zheng1; Mo, Jiankun2; Dong, Xiuling3; Qin, Jianhua1; Lin, Bingcheng1 |
| 刊名 | electrophoresis
 |
| 出版日期 | 2007-12-01 |
| 卷号 | 28期号:24页码:4741-4747 |
| 关键词 | cell electrophoresis K562 cells microfluidic chip multipoint detection |
| 产权排序 | 1;1 |
| 通讯作者 | 林炳承 ; 秦建华 |
| 英文摘要 | in this work, the electrophoretic mobility (epm) measurement of individual cells was investigated by a simple on-chip electrophoresis system with lif multipoint detection. the system enabled the characterization of cell electrophoresis behavior as well as the fluorescence signal from individual cells simultaneously. the measurement yielded the electropherograms of a large number of cells labeled with dye, in which the migration time and migration distance could be obtained easily. the epm has been demonstrated to be different between the k562 cells and k562 cells treated with anticancer drug arsenic trioxide (as(2)o(3)). the k562 cells were found to exhibit a lower epm compared to the cells after drug addition with different concentration. in this preliminary study, over 300 cells could be analyzed within 2 h, demonstrated a much higher analysis throughput compared with traditional methods. the established system is simple and fast, which is expected to be a promising method for evaluating cell surface properties and to be useful in clinical and pharmaceutical applications. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | red-blood-cells ; arsenic trioxide ; apoptosis ; adsorption ; cytometry ; mobility ; flow |
| 收录类别 | SCI |
| 原文出处 | true |
| 语种 | 英语 |
| WOS记录号 | WOS:000252465600026 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/99833]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Biotechnol, Dalian 116023, Peoples R China 2.2nd Peoples Hosp Guangdong Prov, Guangdong, Peoples R China 3.Dalian Third Municipal Hosp, Dalian, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yu, Linfen,Shen, Zheng,Mo, Jiankun,et al. Microfluidic chip-based cell electrophoresis with multipoint laser-induced fluorescence detection system[J]. electrophoresis,2007,28(24):4741-4747. |
| APA | Yu, Linfen,Shen, Zheng,Mo, Jiankun,Dong, Xiuling,Qin, Jianhua,&Lin, Bingcheng.(2007).Microfluidic chip-based cell electrophoresis with multipoint laser-induced fluorescence detection system.electrophoresis,28(24),4741-4747. |
| MLA | Yu, Linfen,et al."Microfluidic chip-based cell electrophoresis with multipoint laser-induced fluorescence detection system".electrophoresis 28.24(2007):4741-4747. |
入库方式： OAI收割
来源：大连化学物理研究所
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