Online multidimensional separation with biphasic monolithic capillary column for shotgun proteome analysis
文献类型：期刊论文
| 作者 | Wang, Fangjun; Dong, Jing; Ye, Mingliang; Jiang, Xiaogang; Wu, Ren'an; Zou, Hanfa |
| 刊名 | journal of proteome research
 |
| 出版日期 | 2008 |
| 卷号 | 7期号:1页码:306-310 |
| 关键词 | biphasic monolithic column shotgun proteome analysis multidimensional separation mu HPLC-MS/MS |
| ISSN号 | 1535-3893 |
| 产权排序 | 1;1 |
| 通讯作者 | 邹汉法 |
| 英文摘要 | a biphasic monolithic capillary column with 10 cm segment of strong-cation exchange monolith and 65 cm segment of reversed-phase monolith was prepared within a single 100 mu m i.d. capillary. separation performance of this column was evaluated by a five-cycle online multidimensional separation of 10 mu g tryptic digest of yeast proteins using nanoflow liquid chromatography coupled with tandem mass spectrometry, and it took 12 h for whole separation under the operating pressure only similar to 900 psi. totally, 780 distinct proteins were positively identified through assignment of 2953 unique peptides at false-positive rate less than 1%. the good separation performance of this biphasic column was largely attributed to the good orthogonality of the strong-cation exchange monolith and reversed-phase monolith for multidimensional separation. |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 类目[WOS] | biochemical research methods |
| 研究领域[WOS] | biochemistry & molecular biology |
| 关键词[WOS] | performance liquid-chromatography ; organic polymer monoliths ; mass-spectrometry ; identification technology ; membrane-proteins ; stationary phases ; yeast proteome ; trap column ; peptides ; samples |
| 收录类别 | SCI |
| 原文出处 | true |
| 语种 | 英语 |
| WOS记录号 | WOS:000252154200036 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/99861]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Fangjun,Dong, Jing,Ye, Mingliang,et al. Online multidimensional separation with biphasic monolithic capillary column for shotgun proteome analysis[J]. journal of proteome research,2008,7(1):306-310. |
| APA | Wang, Fangjun,Dong, Jing,Ye, Mingliang,Jiang, Xiaogang,Wu, Ren'an,&Zou, Hanfa.(2008).Online multidimensional separation with biphasic monolithic capillary column for shotgun proteome analysis.journal of proteome research,7(1),306-310. |
| MLA | Wang, Fangjun,et al."Online multidimensional separation with biphasic monolithic capillary column for shotgun proteome analysis".journal of proteome research 7.1(2008):306-310. |
入库方式： OAI收割
来源：大连化学物理研究所
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