Miniaturized two-dimensional capillary electrophoresis on a microchip for analysis of the tryptic digest of proteins
文献类型：期刊论文
| 作者 | Cong, Yongzheng1,2; Zhang, Lihua1; Tao, Dingyin1; Liang, Yu1; Zhang, Weibing1; Zhang, Yukui1 |
| 刊名 | journal of separation science
 |
| 出版日期 | 2008-02-01 |
| 卷号 | 31期号:3页码:588-594 |
| 关键词 | microchip electrophoresis peptides permanent coating separation voltage two dimensional electrophoresis |
| 产权排序 | 1;1 |
| 通讯作者 | 张丽华 |
| 英文摘要 | a two-dimensional capillary electrophoresis platform, combining isoelectric focusing (ief) and capillary zone electrophoresis (cze), was established on a microchip with the channel width and depth as 100 mu m and 40 mu m, respectively. with polyacrylamide as permanent coating, eof in the microchannel, which could impair the separation, was decreased to 3.4 x 10(-9) m(2) . v(-1) . s(-1), about 1/10 of that obtained in the uncoated set-up. during the separation, peptides were first focused by ief in the first dimensional channel, and then directly driven into the perpendicular channel by controlling the applied voltages, and separated by cze. effects of various experimental parameters, including the electric field strength, channel length, and injection frequency from the first to the second dimensional separation channel, were studied. under optimized condition, the digests of bsa and proteins extracted from e. coli were separated, and a peak capacity of 540 was obtained, which was far greater than that obtained by each single dimensional separation. all these results showed the promise of multidimensional separation on a microchip for the high-throughput and high-resolution analysis of complex samples. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, analytical |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | isotachophoresis-zone electrophoresis ; electroosmotic flow ; gel-electrophoresis ; microfluidic chips ; high-efficiency ; separation ; preconcentration ; chromatography ; systems ; device |
| 收录类别 | SCI |
| 原文出处 | true |
| 语种 | 英语 |
| WOS记录号 | WOS:000253689700018 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/99923]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Cong, Yongzheng,Zhang, Lihua,Tao, Dingyin,et al. Miniaturized two-dimensional capillary electrophoresis on a microchip for analysis of the tryptic digest of proteins[J]. journal of separation science,2008,31(3):588-594. |
| APA | Cong, Yongzheng,Zhang, Lihua,Tao, Dingyin,Liang, Yu,Zhang, Weibing,&Zhang, Yukui.(2008).Miniaturized two-dimensional capillary electrophoresis on a microchip for analysis of the tryptic digest of proteins.journal of separation science,31(3),588-594. |
| MLA | Cong, Yongzheng,et al."Miniaturized two-dimensional capillary electrophoresis on a microchip for analysis of the tryptic digest of proteins".journal of separation science 31.3(2008):588-594. |
入库方式： OAI收割
来源：大连化学物理研究所
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