Cryopreservation of sperm from Atlantic halibut (Hippoglossus hippoglossus, L.) for commercial application
文献类型：期刊论文
| 作者 | Ding, Fuhong1; Lall, Santosh P.2; Li, Jun3; Lei, Jilin1; Rommens, Melissa4; Milley, Joyce E.2 |
| 刊名 | CRYOBIOLOGY
 |
| 出版日期 | 2011-08-01 |
| 卷号 | 63期号:1页码:56-60 |
| 关键词 | Atlantic Halibut Cryopreservation Hippoglossus Hippoglossus Sperm L. Fertilization Motility |
| ISSN号 | 0011-2240 |
| DOI | 10.1016/j.cryobiol.2011.04.009 |
| 文献子类 | Article |
| 英文摘要 | Development of Atlantic halibut (Hippoglossus hippoglossus) aquaculture will be enhanced with cryopreservation of halibut sperm by ensuring a reliable supply of sperm of desired quality and quantity. To assist in its commercial application, the cryopreservation of large volumes of halibut sperm was investigated. Three cryoprotectants were compared: dimethyl sulfoxide (DMSO), polyethylene glycol (PG) and glycerol (GLY) at two concentrations (10% or 15%). Two salt solutions, Hanks' balanced salt solution (HBSS) and 0.1 M KHCO(3) with 0.125 M sucrose solution (KS) were tested as diluents. Both factors were examined in 1.6 mL volumes. A cryopreservation volume of 4 mL and a low dilution ratio (1:1) were examined separately. Based on motility and fertilization rate, 10% and 15% DMSO diluted with HBSS or KS solution proved to be effective extenders with mean fertilization rates ranging from 52.2 +/- 27.2% to 65.8 +/- 26.1%; none of which were significantly different from that of the control. Four other extenders, 10% PG or 10% GLY with HBSS or KS, resulted in significantly lower fertilization rates. Use of a 4 mL cryopreservation volume did not exhibit a significant effect on fertilization rate or motility of post-thawed sperm compared to a 1.6 mL volume (P > 0.05); while the use of a dilution ratio of one part sperm with three parts ciyopreservation solution (1:3 v/v with sperm concentration of 0.51 +/- 0.11 x 10(10) cells/ml) had a significantly better preservation effect than using a ratio of 1:1 with sperm concentration of 1.02 +/- 0.21 x 10(10) cells/ml (P < 0.05). From these results, an optimized protocol for the cryopreservation of Atlantic halibut sperm using a volume as large as 4 mL has been established. (C) 2011 Elsevier Inc. All rights reserved.; Development of Atlantic halibut (Hippoglossus hippoglossus) aquaculture will be enhanced with cryopreservation of halibut sperm by ensuring a reliable supply of sperm of desired quality and quantity. To assist in its commercial application, the cryopreservation of large volumes of halibut sperm was investigated. Three cryoprotectants were compared: dimethyl sulfoxide (DMSO), polyethylene glycol (PG) and glycerol (GLY) at two concentrations (10% or 15%). Two salt solutions, Hanks' balanced salt solution (HBSS) and 0.1 M KHCO(3) with 0.125 M sucrose solution (KS) were tested as diluents. Both factors were examined in 1.6 mL volumes. A cryopreservation volume of 4 mL and a low dilution ratio (1:1) were examined separately. Based on motility and fertilization rate, 10% and 15% DMSO diluted with HBSS or KS solution proved to be effective extenders with mean fertilization rates ranging from 52.2 +/- 27.2% to 65.8 +/- 26.1%; none of which were significantly different from that of the control. Four other extenders, 10% PG or 10% GLY with HBSS or KS, resulted in significantly lower fertilization rates. Use of a 4 mL cryopreservation volume did not exhibit a significant effect on fertilization rate or motility of post-thawed sperm compared to a 1.6 mL volume (P > 0.05); while the use of a dilution ratio of one part sperm with three parts ciyopreservation solution (1:3 v/v with sperm concentration of 0.51 +/- 0.11 x 10(10) cells/ml) had a significantly better preservation effect than using a ratio of 1:1 with sperm concentration of 1.02 +/- 0.21 x 10(10) cells/ml (P < 0.05). From these results, an optimized protocol for the cryopreservation of Atlantic halibut sperm using a volume as large as 4 mL has been established. (C) 2011 Elsevier Inc. All rights reserved. |
| 学科主题 | Life Sciences & Biomedicine - Other Topics ; Physiology |
| URL标识 | 查看原文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000292020800009 |
| 公开日期 | 2012-07-03 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/12008]  |
| 专题 | 海洋研究所_海洋生物技术研发中心 |
| 作者单位 | 1.Chinese Acad Fishery Sci, Yellowsea Fisheries Res Inst, Qingdao 266071, Peoples R China 2.Natl Res Council Canada, Inst Marine Biosci, Halifax, NS B3H 3Z1, Canada 3.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 4.Scotian Halibut Ltd, Clarks Harbour, NS B0W 1P0, Canada |
| 推荐引用方式 GB/T 7714 | Ding, Fuhong,Lall, Santosh P.,Li, Jun,et al. Cryopreservation of sperm from Atlantic halibut (Hippoglossus hippoglossus, L.) for commercial application[J]. CRYOBIOLOGY,2011,63(1):56-60. |
| APA | Ding, Fuhong,Lall, Santosh P.,Li, Jun,Lei, Jilin,Rommens, Melissa,&Milley, Joyce E..(2011).Cryopreservation of sperm from Atlantic halibut (Hippoglossus hippoglossus, L.) for commercial application.CRYOBIOLOGY,63(1),56-60. |
| MLA | Ding, Fuhong,et al."Cryopreservation of sperm from Atlantic halibut (Hippoglossus hippoglossus, L.) for commercial application".CRYOBIOLOGY 63.1(2011):56-60. |
入库方式： OAI收割
来源：海洋研究所
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