STUDY ON PERMEABILITY OF DMSO IN EMBRYOS OF RED SEABREAM (PAGRUS MAJOR) BY CAPILLARY ELECTROPHORESIS
文献类型：期刊论文
| 作者 | Lin, F.1,2; Liu, Q. H.1; Xiao, Z. Z.1; Ma, D. Y.1; Xu, S. H.; Xiao, Y. S.; Li, J.1
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| 刊名 | CRYOLETTERS
 |
| 出版日期 | 2011-07-01 |
| 卷号 | 32期号:4页码:339-348 |
| 关键词 | Pagrus Major Dmso Permeability Capillary Electrophoresis Hatching Rate Morphology Changes |
| ISSN号 | 0143-2044 |
| 文献子类 | Article |
| 英文摘要 | The objectives were to investigate the permeability of DMSO to red seabream (Pagrus major) embryos by capillary electrophoresis and the effects of DMSO concentrations (5%-40%, v/v) and immersion times (10, 30 and 60 min) on hatching rate and morphology. The results suggested the internal DMSO concentrations were positively related with the external concentrations and exposure times, while the hatching rate was negatively related. The hatching rate decreased drastically (< 50%) after exposure in 35%, 20% and 15% DMSO for over 10, 30 and 60 min, respectively. In all groups, when hatching rate was > 50%, the internal DMSO concentration was < 2%, which was still insufficient for successful cryopreservation. Morphological changes indicated the chorion was permeable to the cryoprotectant. A sign of dehydration in yolk were observed, for a significant decrease in the maximal yolk sac diameter. However, further research was needed to investigate whether the DMSO permeated into the yolk.; The objectives were to investigate the permeability of DMSO to red seabream (Pagrus major) embryos by capillary electrophoresis and the effects of DMSO concentrations (5%-40%, v/v) and immersion times (10, 30 and 60 min) on hatching rate and morphology. The results suggested the internal DMSO concentrations were positively related with the external concentrations and exposure times, while the hatching rate was negatively related. The hatching rate decreased drastically (< 50%) after exposure in 35%, 20% and 15% DMSO for over 10, 30 and 60 min, respectively. In all groups, when hatching rate was > 50%, the internal DMSO concentration was < 2%, which was still insufficient for successful cryopreservation. Morphological changes indicated the chorion was permeable to the cryoprotectant. A sign of dehydration in yolk were observed, for a significant decrease in the maximal yolk sac diameter. However, further research was needed to investigate whether the DMSO permeated into the yolk. |
| 学科主题 | Life Sciences & Biomedicine - Other Topics ; Physiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000295308200006 |
| 公开日期 | 2012-07-03 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/12060]  |
| 专题 | 海洋研究所_海洋生物技术研发中心 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Ctr Biotechnol R&D, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Lin, F.,Liu, Q. H.,Xiao, Z. Z.,et al. STUDY ON PERMEABILITY OF DMSO IN EMBRYOS OF RED SEABREAM (PAGRUS MAJOR) BY CAPILLARY ELECTROPHORESIS[J]. CRYOLETTERS,2011,32(4):339-348. |
| APA | Lin, F..,Liu, Q. H..,Xiao, Z. Z..,Ma, D. Y..,Xu, S. H..,...&Li, J..(2011).STUDY ON PERMEABILITY OF DMSO IN EMBRYOS OF RED SEABREAM (PAGRUS MAJOR) BY CAPILLARY ELECTROPHORESIS.CRYOLETTERS,32(4),339-348. |
| MLA | Lin, F.,et al."STUDY ON PERMEABILITY OF DMSO IN EMBRYOS OF RED SEABREAM (PAGRUS MAJOR) BY CAPILLARY ELECTROPHORESIS".CRYOLETTERS 32.4(2011):339-348. |
入库方式： OAI收割
来源：海洋研究所
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