Tandem duplication-random loss is not a real feature of oyster mitochondrial genomes
文献类型：期刊论文
| 作者 | Ren, Jianfeng; Liu, Xiao; Zhang, Guofan; Liu, Bin; Guo, Ximing; Liu, B, Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China |
| 刊名 | BMC GENOMICS
 |
| 出版日期 | 2009 |
| 卷号 | 10页码:84 |
| 关键词 | Dna Sequence Crassostrea |
| ISSN号 | 1471-2164 |
| 英文摘要 | Duplications and rearrangements of coding genes are major themes in the evolution of mitochondrial genomes, bearing important consequences in the function of mitochondria and the fitness of organisms. Yu et al. (BMC Genomics 2008, 9: 477) reported the complete mt genome sequence of the oyster Crassostrea hongkongensis (16,475 bp) and found that a DNA segment containing four tRNA genes (trnK(1), trnC, trnQ(1) and trnN), a duplicated (rrnS) and a split rRNA gene (rrnL5') was absent compared with that of two other Crassostrea species. It was suggested that the absence was a novel case of "tandem duplication-random loss" with evolutionary significance. We independently sequenced the complete mt genome of three C. hongkongensis individuals, all of which were 18,622 bp and contained the segment that was missing in Yu et al.'s sequence. Further, we designed primers, verified sequences and demonstrated that the sequence loss in Yu et al.'s study was an artifact caused by placing primers in a duplicated region. The duplication and split of ribosomal RNA genes are unique for Crassostrea oysters and not lost in C. hongkongensis. Our study highlights the need for caution when amplifying and sequencing through duplicated regions of the genome. |
| 学科主题 | Biotechnology & Applied Microbiology ; Genetics & Heredity |
| URL标识 | 查看原文 |
| 公开日期 | 2010-11-18 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/1871]  |
| 专题 | 海洋研究所_海洋生物技术研发中心 |
| 通讯作者 | Liu, B, Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ren, Jianfeng,Liu, Xiao,Zhang, Guofan,et al. Tandem duplication-random loss is not a real feature of oyster mitochondrial genomes[J]. BMC GENOMICS,2009,10:84. |
| APA | Ren, Jianfeng,Liu, Xiao,Zhang, Guofan,Liu, Bin,Guo, Ximing,&Liu, B, Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China.(2009).Tandem duplication-random loss is not a real feature of oyster mitochondrial genomes.BMC GENOMICS,10,84. |
| MLA | Ren, Jianfeng,et al."Tandem duplication-random loss is not a real feature of oyster mitochondrial genomes".BMC GENOMICS 10(2009):84. |
入库方式： OAI收割
来源：海洋研究所
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