Purification and characterization of a novel glycosidase from the China white jade snail (Achatina fulica) showing transglycosylation activity
文献类型：期刊论文
| 作者 | Hu, Ying1,2; Luan, Hongwei1,2; Zhou, Kun1,2; Ge, Guangbo1,2; Yang, Shengli1; Yang, Ling1 |
| 刊名 | enzyme and microbial technology
 |
| 出版日期 | 2008-07-07 |
| 卷号 | 43期号:1页码:35-42 |
| 关键词 | beta-D-glycosidase transglycosylation thermal and pH stability broad specificity alkyl glycosides enzyme purification |
| 产权排序 | 1;1 |
| 通讯作者 | 杨凌 |
| 英文摘要 | a broad specificity beta-d-glycosidase, designated g1, was purified to homogeneity from the viscera of the china white jade snail (achatina fulica). the enzyme was a monomeric protein with molecular weight of 115 kda. g i has broad glycone specificity towards p-nitrophenyl derivatives of beta-d-glucose, beta-d-fucose, beta-d-galactose, alpha-d-glucose and some disaccharides. the optimum ph and temperature are 5.5 and 55 degrees c, respectively. it was stable over a wide ph range (5-10 at 30 degrees c for 24h) and against a relatively high temperature (50 degrees c for 4h). moreover, it was also stable and active in the presence of various alcohols. with pnpglu, pnpfuc and cellobiose as donor, g i showed high transglycosylation activity. six transglycosylation products were isolated from the reaction mixture containing 20% alcohol as glycoside acceptor using a preparative thin layer chromatography (preparative tlc) and identified as alkyl-glucos ides and alkyl-fucosides by mass spectrometry (ms) analysis. combining the high alcohol tolerance, moderate temperature and ph stability and alkyl glycosides production efficiency through transglycosylation, g i can be considered to be a promising candidate for the production of various alkyl glycosides. (c) 2008 elsevier inc. all rights reserved. |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 类目[WOS] | biotechnology & applied microbiology |
| 研究领域[WOS] | biotechnology & applied microbiology |
| 关键词[WOS] | beta-d-glucosidase ; catalyzed condensation ; thermophilic fungus ; escherichia-coli ; alkyl glucosides ; biphasic system ; galactosidase ; fucosidase ; hydrolase ; donor |
| 收录类别 | SCI |
| 原文出处 | true |
| 语种 | 英语 |
| WOS记录号 | WOS:000257154400006 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/100019]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Pharmaceut Resource Discovery, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Hu, Ying,Luan, Hongwei,Zhou, Kun,et al. Purification and characterization of a novel glycosidase from the China white jade snail (Achatina fulica) showing transglycosylation activity[J]. enzyme and microbial technology,2008,43(1):35-42. |
| APA | Hu, Ying,Luan, Hongwei,Zhou, Kun,Ge, Guangbo,Yang, Shengli,&Yang, Ling.(2008).Purification and characterization of a novel glycosidase from the China white jade snail (Achatina fulica) showing transglycosylation activity.enzyme and microbial technology,43(1),35-42. |
| MLA | Hu, Ying,et al."Purification and characterization of a novel glycosidase from the China white jade snail (Achatina fulica) showing transglycosylation activity".enzyme and microbial technology 43.1(2008):35-42. |
入库方式： OAI收割
来源：大连化学物理研究所
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