Comparative analysis of methods for concentrating venom from jellyfish Rhopilema esculentum Kishinouye
文献类型：期刊论文
| 作者 | Li Cuiping1,2; Yu Huahua1 ; Feng Jinhua1,2; Chen Xiaolin1,2; Li Pengcheng1
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| 刊名 | CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
 |
| 出版日期 | 2009-02-01 |
| 卷号 | 27期号:1页码:172-176 |
| 关键词 | Concentration Hemolytic Activity Rhopilema Esculentum Kishinouye Venom |
| ISSN号 | 0254-4059 |
| DOI | 10.1007/s00343-009-0172-2 |
| 文献子类 | Article |
| 英文摘要 | In this study, several methods were compared for the efficiency to concentrate venom from the tentacles of jellyfish Rhopilema esculentum Kishinouye. The results show that the methods using either freezing-dry or gel absorption to remove water to concentrate venom are not applicable due to the low concentration of the compounds dissolved. Although the recovery efficiency and the total venom obtained using the dialysis dehydration method are high, some proteins can be lost during the concentrating process. Comparing to the lyophilization method, ultrafiltration is a simple way to concentrate the compounds at high percentage but the hemolytic activities of the proteins obtained by ultrafiltration appear to be lower. Our results suggest that overall lyophilization is the best and recommended method to concentrate venom from the tentacles of jellyfish. It shows not only the high recovery efficiency for the venoms but high hemolytic activities as well.; In this study, several methods were compared for the efficiency to concentrate venom from the tentacles of jellyfish Rhopilema esculentum Kishinouye. The results show that the methods using either freezing-dry or gel absorption to remove water to concentrate venom are not applicable due to the low concentration of the compounds dissolved. Although the recovery efficiency and the total venom obtained using the dialysis dehydration method are high, some proteins can be lost during the concentrating process. Comparing to the lyophilization method, ultrafiltration is a simple way to concentrate the compounds at high percentage but the hemolytic activities of the proteins obtained by ultrafiltration appear to be lower. Our results suggest that overall lyophilization is the best and recommended method to concentrate venom from the tentacles of jellyfish. It shows not only the high recovery efficiency for the venoms but high hemolytic activities as well. |
| 学科主题 | Limnology ; Oceanography |
| URL标识 | 查看原文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000264616100027 |
| 公开日期 | 2010-12-22 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/3473]  |
| 专题 | 海洋研究所_海洋生物技术研发中心 海洋研究所_海洋环境工程技术研究发展中心 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li Cuiping,Yu Huahua,Feng Jinhua,et al. Comparative analysis of methods for concentrating venom from jellyfish Rhopilema esculentum Kishinouye[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2009,27(1):172-176. |
| APA | Li Cuiping,Yu Huahua,Feng Jinhua,Chen Xiaolin,&Li Pengcheng.(2009).Comparative analysis of methods for concentrating venom from jellyfish Rhopilema esculentum Kishinouye.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,27(1),172-176. |
| MLA | Li Cuiping,et al."Comparative analysis of methods for concentrating venom from jellyfish Rhopilema esculentum Kishinouye".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 27.1(2009):172-176. |
入库方式： OAI收割
来源：海洋研究所
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