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Inheritance of 15 microsatellites in the Pacific oyster Crassostrea gigas: segregation and null allele identification for linkage analysis

文献类型：期刊论文

作者Li Li1; Guo Ximing2; Zhang Guofan1
刊名CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
出版日期2009-02-01
卷号27期号:1页码:74-79
关键词Crassostrea Gigas Microsatellite Null Allele Genetic Mapping
ISSN号0254-4059
DOI10.1007/s00343-009-0074-3
文献子类Article
英文摘要Microsatellites were screened in a backcross family of the Pacific oyster, Crassostrea gigas. Fifteen microsatellite loci were distinguishable and polymorphic with 6 types of allele-combinations. Null alleles were detected in 46.7% of loci, accounting for 11.7% of the total alleles. Four loci did not segregate in Mendelian Ratios. Three linkage groups were identified among 7 of the 15 segregating loci. Fluorescence-based automated capillary electrophoresis (ABI 310 Genetic Analyzer) that used to detect the microsatellite loci, has been proved a fast, precise, and reliable method in microsatellite genotyping.; Microsatellites were screened in a backcross family of the Pacific oyster, Crassostrea gigas. Fifteen microsatellite loci were distinguishable and polymorphic with 6 types of allele-combinations. Null alleles were detected in 46.7% of loci, accounting for 11.7% of the total alleles. Four loci did not segregate in Mendelian Ratios. Three linkage groups were identified among 7 of the 15 segregating loci. Fluorescence-based automated capillary electrophoresis (ABI 310 Genetic Analyzer) that used to detect the microsatellite loci, has been proved a fast, precise, and reliable method in microsatellite genotyping.
学科主题Limnology ; Oceanography
URL标识查看原文
语种英语
WOS记录号WOS:000264616100013
公开日期2010-12-22
源URL[http://ir.qdio.ac.cn/handle/337002/3475]  
专题海洋研究所_海洋生物技术研发中心
作者单位1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Rutgers State Univ, Inst Marine & Coastal Sci, Haskin Shellfish Res Lab, New Brunswick, NJ 08349 USA
推荐引用方式
GB/T 7714		 Li Li,Guo Ximing,Zhang Guofan. Inheritance of 15 microsatellites in the Pacific oyster Crassostrea gigas: segregation and null allele identification for linkage analysis[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2009,27(1):74-79.
APA Li Li,Guo Ximing,&Zhang Guofan.(2009).Inheritance of 15 microsatellites in the Pacific oyster Crassostrea gigas: segregation and null allele identification for linkage analysis.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,27(1),74-79.
MLA Li Li,et al."Inheritance of 15 microsatellites in the Pacific oyster Crassostrea gigas: segregation and null allele identification for linkage analysis".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 27.1(2009):74-79.

入库方式： OAI收割

来源：海洋研究所

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