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Factors affecting the protease activity of venom from jellyfish Rhopilema esculentum Kishinouye

文献类型：期刊论文

作者Li, CP; Yu, HH; Liu, S; Xing, RE; Guo, ZY; Li, PC
刊名BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
出版日期2005-12-15
卷号15期号:24页码:5370-5374
关键词Jellyfish Protease Activity Rhopilema Esculentum Kishinouye Venom
ISSN号0960-894X
DOI10.1016/j.bmcl.2005.09.010
文献子类Article
英文摘要In this paper, the effects of some chemical and physical factors such as temperature, pH values, glycerol, and divalent metal cations on the protease activity of venom from jellyfish, Rhopilema esculentum Kishinouye, were assayed. Protease activity was dependent on temperature and pH values. Zn(2+), Mg(2+), and Mn(2+) in sodium phosphate buffer (0.02 M, pH 8.0) could increase protease activity. Mn(2+) had the best effects among the three metal cations and the effect was about 20 times of that of Zn(2+) or Mg(2+) and its maximal protease activity was 2.3 x 10(5) U/mL. EDTA could increase protease activity. PMSF had hardly affected protease activity. O-Phenanthroline and glycerol played an important part in inhibiting protease activity and their maximal inhibiting rates were 87.5% and 82.1%, respectively. (c) 2005 Elsevier Ltd. All rights reserved.; In this paper, the effects of some chemical and physical factors such as temperature, pH values, glycerol, and divalent metal cations on the protease activity of venom from jellyfish, Rhopilema esculentum Kishinouye, were assayed. Protease activity was dependent on temperature and pH values. Zn2+, Mg2+, and Mn2+ in sodium phosphate buffer (0.02 M, pH 8.0) could increase protease activity. Mn2+ had the best effects among the three metal cations and the effect was about 20 times of that of Zn2+ or Mg2+ and its maximal protease activity was 2.3 x 10(5) U/mL. EDTA could increase protease activity. PMSF had hardly affected protease activity. O-Phenanthroline and glycerol played an important part in inhibiting protease activity and their maximal inhibiting rates were 87.5% and 82.1%, respectively. (c) 2005 Elsevier Ltd. All rights reserved.
学科主题Chemistry, Medicinal ; Chemistry, Organic
URL标识查看原文
语种英语
WOS记录号WOS:000233206300002
公开日期2010-12-22
源URL[http://ir.qdio.ac.cn/handle/337002/3515]  
专题海洋研究所_海洋生物技术研发中心
海洋研究所_海洋环境工程技术研究发展中心
作者单位1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
推荐引用方式
GB/T 7714		 Li, CP,Yu, HH,Liu, S,et al. Factors affecting the protease activity of venom from jellyfish Rhopilema esculentum Kishinouye[J]. BIOORGANIC & MEDICINAL CHEMISTRY LETTERS,2005,15(24):5370-5374.
APA Li, CP,Yu, HH,Liu, S,Xing, RE,Guo, ZY,&Li, PC.(2005).Factors affecting the protease activity of venom from jellyfish Rhopilema esculentum Kishinouye.BIOORGANIC & MEDICINAL CHEMISTRY LETTERS,15(24),5370-5374.
MLA Li, CP,et al."Factors affecting the protease activity of venom from jellyfish Rhopilema esculentum Kishinouye".BIOORGANIC & MEDICINAL CHEMISTRY LETTERS 15.24(2005):5370-5374.

入库方式： OAI收割

来源：海洋研究所

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