One step isolation and purification of liquiritigenin and isoliquiritigenin from Glycyrrhiza uralensis Risch using high-speed counter-current chromatography
文献类型：期刊论文
| 作者 | Ma, CJ; Li, GS; Zhang, DL; Liu, K ; Fan, X
|
| 刊名 | JOURNAL OF CHROMATOGRAPHY A
 |
| 出版日期 | 2005-06-17 |
| 卷号 | 1078期号:1-2页码:188-192 |
| 关键词 | Counter-current Chromatography Glycyrrhiza Uralensis Plant Materials Pharmaceutical Analysis Liquiritigenin Isoliquiritigenin |
| ISSN号 | 0021-9673 |
| DOI | 10.1016/j.chroma.2005.01.053 |
| 文献子类 | Article |
| 英文摘要 | High-speed counter-current chromatography (HSCCC) technique in semi-preparative scale has been successfully applied to the separation of bioactive flavonoid compounds, liquiritigenin and isoliquiritigenin in one step from the crude extract of Glycyrrhiza uralensis Risch. The HSCCC was performed using a two-phase solvent system composed of n-hexane-ethyl acetate-methanol-acetonitrile-water (2:2:1:0.6:2, v/v). Yields of liquiritigenin (98.9% purity) and isoliquiritigenin (98.3% purity) obtained were 0.52% and 0.32%. Chemical structures of the purified liquiritigenin and isoliquiritigenin were identified by electrospray ionization-MS (ESI-MS) and NMR analysis. (c) 2005 Published by Elsevier B.V.; High-speed counter-current chromatography (HSCCC) technique in semi-preparative scale has been successfully applied to the separation of bioactive flavonoid compounds, liquiritigenin and isoliquiritigenin in one step from the crude extract of Glycyrrhiza uralensis Risch. The HSCCC was performed using a two-phase solvent system composed of n-hexane-ethyl acetate-methanol-acetonitrile-water (2:2:1:0.6:2, v/v). Yields of liquiritigenin (98.9% purity) and isoliquiritigenin (98.3% purity) obtained were 0.52% and 0.32%. Chemical structures of the purified liquiritigenin and isoliquiritigenin were identified by electrospray ionization-MS (ESI-MS) and NMR analysis. (c) 2005 Published by Elsevier B.V. |
| 学科主题 | Biochemical Research Methods ; Chemistry, Analytical |
| URL标识 | 查看原文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000229996100024 |
| 公开日期 | 2010-12-22 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/3525]  |
| 专题 | 海洋研究所_海洋生物技术研发中心 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 3.Shandong Engn Res Ctr Nat Drugs, Yantai 264003, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ma, CJ,Li, GS,Zhang, DL,et al. One step isolation and purification of liquiritigenin and isoliquiritigenin from Glycyrrhiza uralensis Risch using high-speed counter-current chromatography[J]. JOURNAL OF CHROMATOGRAPHY A,2005,1078(1-2):188-192. |
| APA | Ma, CJ,Li, GS,Zhang, DL,Liu, K,&Fan, X.(2005).One step isolation and purification of liquiritigenin and isoliquiritigenin from Glycyrrhiza uralensis Risch using high-speed counter-current chromatography.JOURNAL OF CHROMATOGRAPHY A,1078(1-2),188-192. |
| MLA | Ma, CJ,et al."One step isolation and purification of liquiritigenin and isoliquiritigenin from Glycyrrhiza uralensis Risch using high-speed counter-current chromatography".JOURNAL OF CHROMATOGRAPHY A 1078.1-2(2005):188-192. |
入库方式： OAI收割
来源：海洋研究所
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