Effects of nitrogen source and concentration on growth rate and fatty acid composition of Ellipsoidion sp (Eustigmatophyta)
文献类型：期刊论文
| 作者 | Xu, NJ; Zhang, XC; Fan, X; Han, LJ; Zeng, CK; Tseng, CK |
| 刊名 | JOURNAL OF APPLIED PHYCOLOGY
 |
| 出版日期 | 2001-12-01 |
| 卷号 | 13期号:6页码:463-469 |
| 关键词 | Ammonium Urea Eicosapentaenoic Acid Fatty Acid Ellipsoidion Sp. Microalga Nitrate Nitrogen Concentration Nitrogen Source Pufa |
| ISSN号 | 0921-8971 |
| 文献子类 | Article |
| 英文摘要 | In order to study the effects of different nitrogen source and concentration on the growth rate and fatty acid composition, a marine microalga Ellipsoidion sp. with a high content of eicosapentaenoic acid (EPA) was cultured in media with different nitrogen sources and concentrations. During the pre-logarithmic phase, the alga grew faster with ammonium as N source than with nitrate, but the reverse applied during the post-logarithmic phase. The alga grew poorly in N-free medium or medium with urea as the sole N source. In the same growth phase, ammonium medium resulted in higher yield of total lipid, but the EPA yield did not differ significantly different from that using nitrate medium. The maximum growth rate occurred in medium containing 1.28 mmol L-1 sodium nitrate, while maximum EPA and total lipid contents were reached at 1.92 mmol L-1, when EPA accounted for 27.9% total fatty acids. The growth rate kept stable when NH4Cl ranged from 0.64 to 2.56 mmol L-1, and the maximum content of total lipid and EPA occurred in the medium with 2.56 mmol L-1 NH4Cl. The EPA content was higher in the pre- than post-logarithmic phase, though the total lipid content was lower. The highest EPA content expressed as percent total fatty acid was 27.9% in nitrate medium and and 39.0% in ammonium medium.; In order to study the effects of different nitrogen source and concentration on the growth rate and fatty acid composition, a marine microalga Ellipsoidion sp. with a high content of eicosapentaenoic acid (EPA) was cultured in media with different nitrogen sources and concentrations. During the pre-logarithmic phase, the alga grew faster with ammonium as N source than with nitrate, but the reverse applied during the post-logarithmic phase. The alga grew poorly in N-free medium or medium with urea as the sole N source. In the same growth phase, ammonium medium resulted in higher yield of total lipid, but the EPA yield did not differ significantly different from that using nitrate medium. The maximum growth rate occurred in medium containing 1.28 mmol L-1 sodium nitrate, while maximum EPA and total lipid contents were reached at 1.92 mmol L-1, when EPA accounted for 27.9% total fatty acids. The growth rate kept stable when NH4Cl ranged from 0.64 to 2.56 mmol L-1, and the maximum content of total lipid and EPA occurred in the medium with 2.56 mmol L-1 NH4Cl. The EPA content was higher in the pre- than post-logarithmic phase, though the total lipid content was lower. The highest EPA content expressed as percent total fatty acid was 27.9% in nitrate medium and and 39.0% in ammonium medium. |
| 学科主题 | Biotechnology & Applied Microbiology ; Marine & Freshwater Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000171944600001 |
| 公开日期 | 2010-12-22 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/3607]  |
| 专题 | 海洋研究所_海洋生物技术研发中心 |
| 作者单位 | 1.Ocean Univ Qingdao, Coll Marine Life Sci, Qingdao 266003, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xu, NJ,Zhang, XC,Fan, X,et al. Effects of nitrogen source and concentration on growth rate and fatty acid composition of Ellipsoidion sp (Eustigmatophyta)[J]. JOURNAL OF APPLIED PHYCOLOGY,2001,13(6):463-469. |
| APA | Xu, NJ,Zhang, XC,Fan, X,Han, LJ,Zeng, CK,&Tseng, CK.(2001).Effects of nitrogen source and concentration on growth rate and fatty acid composition of Ellipsoidion sp (Eustigmatophyta).JOURNAL OF APPLIED PHYCOLOGY,13(6),463-469. |
| MLA | Xu, NJ,et al."Effects of nitrogen source and concentration on growth rate and fatty acid composition of Ellipsoidion sp (Eustigmatophyta)".JOURNAL OF APPLIED PHYCOLOGY 13.6(2001):463-469. |
入库方式： OAI收割
来源：海洋研究所
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