Optimization of seeding density in microencapsulated recombinant CHO cell culture
文献类型：期刊论文
| 作者 | Zhang, Y.1,2; Zhou, J.1,2; Zhang, X.1,2; Yu, W.1; Guo, X.1; Wang, W.1; Ma, X.1 |
| 刊名 | chemical and biochemical engineering quarterly
 |
| 出版日期 | 2008-03-01 |
| 卷号 | 22期号:1页码:105-111 |
| 关键词 | microencapsulation seeding density recombinant CHO cell endostatin |
| 产权排序 | 1;1 |
| 通讯作者 | 马小军 |
| 英文摘要 | microencapsulation technology is an alternative large-scale mammalian cell culture method. the semi-permeable membrane of the microcapsule allows free diffusion of nutrients, oxygen and toxic metabolites to support cell growth, and the microcapsule membrane can protect the cells from the mechanical damage of shear forces associated with agitation and aeration. many polymers have been used to make microcapsules, such as chitosan, polyacrylates, alginate, polyamino, acids, and polyamides. one of these microcapsules is alginate-polylysine-alginate (apa) microcapsule. using the electrostatic droplet generator and polyelectrolyte complexation, we developed apa microcapsules with a diameter of 300 mu m and assessed the effect of the seeding density on microencapsulated recombinant chinese hamster ovary (cho) cell growth, metabolism and endostatin production. the results showed that the cell growth was the best and endostatin production was the highest when the seeding density was 3 . 10(6) cells ml(-1) microcapsule, at which the maximal cell density reached 1.82 . 10(7) cells ml(-1) microcapsule and endostatin production reached 12.3 mu g ml(-1) at day 9. the energy metabolism was more efficient when the seeding density was 3 . 106 cells ml(-1) microcapsule, at which more glucose and glutamine were utilized for biosynthesis and less lactate and ammonium were produced. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; technology |
| 类目[WOS] | biotechnology & applied microbiology ; engineering, chemical |
| 研究领域[WOS] | biotechnology & applied microbiology ; engineering |
| 关键词[WOS] | antibody-production ; mammalian-cells ; hybridoma cells ; metabolism ; growth |
| 收录类别 | SCI |
| 原文出处 | true |
| 语种 | 英语 |
| WOS记录号 | WOS:000255082500011 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/100037]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Biomed Mat Engn, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Y.,Zhou, J.,Zhang, X.,et al. Optimization of seeding density in microencapsulated recombinant CHO cell culture[J]. chemical and biochemical engineering quarterly,2008,22(1):105-111. |
| APA | Zhang, Y..,Zhou, J..,Zhang, X..,Yu, W..,Guo, X..,...&Ma, X..(2008).Optimization of seeding density in microencapsulated recombinant CHO cell culture.chemical and biochemical engineering quarterly,22(1),105-111. |
| MLA | Zhang, Y.,et al."Optimization of seeding density in microencapsulated recombinant CHO cell culture".chemical and biochemical engineering quarterly 22.1(2008):105-111. |
入库方式： OAI收割
来源：大连化学物理研究所
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