Possible suppression of exogenous beta-1,3-glucanase gene gluc78 on rice transformation and growth
文献类型：期刊论文
| 作者 | Liu, Mei; Zhu, Jie; Sun, Zong-Xiu; Xu, Tong |
| 刊名 | PLANT SCIENCE
 |
| 出版日期 | 2007-05-01 |
| 卷号 | 172期号:5页码:888-896 |
| 关键词 | Rice Beta-1 Transformation 3-glucanase Gene Growth Suppression |
| ISSN号 | 0168-9452 |
| DOI | 10.1016/j.plantsci.2006.11.017 |
| 文献子类 | Article |
| 英文摘要 | Three genes encoding for fungal cell wall degrading enzymes (CWDE), ech42, nag7O and gluc78 from the biocontrol fungus Trichoderma atroviride were transformed into rice mediated by Agrobacterium tumefaciens singly and in all possible combinations. A total of more than 1800 independently regenerated plantlets in seven different populations (for each of the three genes and each of the four gene combinations) were obtained. Our data indicated that gluc78 gene had negative effects on transformation frequency and plant growth. Some regenerated plants with gluc78 gene were stunted; spontaneously produced brown specks; could not tassel. The combination with either one of the two other genes (ech42, nag70) present in the same T-DNA region reduced the negative effect of gluc78 on plant growth. These results indicated that expression of several genes in one T-DNA region interfered with each other and expression of exogenous gene in recipient plant was a complex behavior. (c) 2007 Published by Elsevier Ireland Ltd.; Three genes encoding for fungal cell wall degrading enzymes (CWDE), ech42, nag7O and gluc78 from the biocontrol fungus Trichoderma atroviride were transformed into rice mediated by Agrobacterium tumefaciens singly and in all possible combinations. A total of more than 1800 independently regenerated plantlets in seven different populations (for each of the three genes and each of the four gene combinations) were obtained. Our data indicated that gluc78 gene had negative effects on transformation frequency and plant growth. Some regenerated plants with gluc78 gene were stunted; spontaneously produced brown specks; could not tassel. The combination with either one of the two other genes (ech42, nag70) present in the same T-DNA region reduced the negative effect of gluc78 on plant growth. These results indicated that expression of several genes in one T-DNA region interfered with each other and expression of exogenous gene in recipient plant was a complex behavior. (c) 2007 Published by Elsevier Ireland Ltd. |
| 学科主题 | Biochemistry & Molecular Biology ; Plant Sciences |
| URL标识 | 查看原文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000246137400003 |
| 公开日期 | 2010-12-24 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/6069]  |
| 专题 | 海洋研究所_海洋生物技术研发中心 |
| 作者单位 | 1.China Rice Natl Res Inst, State Key Lab Rice Biol, Hangzhou 310006, Peoples R China 2.Zhejiang Univ, Coll Agr & Biotechnol, Hangzhou 310029, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, R&D Ctr Marine Biotechnol, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Mei,Zhu, Jie,Sun, Zong-Xiu,et al. Possible suppression of exogenous beta-1,3-glucanase gene gluc78 on rice transformation and growth[J]. PLANT SCIENCE,2007,172(5):888-896. |
| APA | Liu, Mei,Zhu, Jie,Sun, Zong-Xiu,&Xu, Tong.(2007).Possible suppression of exogenous beta-1,3-glucanase gene gluc78 on rice transformation and growth.PLANT SCIENCE,172(5),888-896. |
| MLA | Liu, Mei,et al."Possible suppression of exogenous beta-1,3-glucanase gene gluc78 on rice transformation and growth".PLANT SCIENCE 172.5(2007):888-896. |
入库方式： OAI收割
来源：海洋研究所
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