Use of computer-assisted sperm analysis (CASA) to evaluate the quality of cryopreserved sperm in red seabream (Pagrus major)
文献类型：期刊论文
| 作者 | Liu, Qing Hua; Li, Jun; Xiao, Zhi Zhong; Ding, Fu Hong; Yu, Dao De; Xu, Xi Zhang |
| 刊名 | AQUACULTURE
 |
| 出版日期 | 2007-03-06 |
| 卷号 | 263期号:1-4页码:20-25 |
| 关键词 | Casa Red Seabream Pagrus Major Sperm Motility Velocity Fertilizing Capacity |
| ISSN号 | 0044-8486 |
| DOI | 10.1016/j.aquaculture.2006.11.017 |
| 文献子类 | Article |
| 英文摘要 | In the present study, the quality of post-thaw sperm of red seabream Pagrus major frozen with 6-24% DMSO was investigated. The motility, average path velocity and fertilizing capacity of fresh and their corresponding post-thaw sperm were examined for evaluation of the post-thaw sperm motion characteristics and its association with fertilizing capacity. An analysis of sperm motility before and after cryopreservation has been performed using computer-assisted sperm analysis (CASA). For post-thaw sperm frozen with 12-21% DMSO, the percentages of motile sperm were not significantly (P > 0.05) changed 10 s after activation. Moreover, the main motility pattern and swimming velocity of the motile post-thaw sperm were not significantly (P > 0.05) changed and the progressive linear motion was still the dominant pattern. However, the total motility of post-thaw sperm (72.3 +/- 6.3%) 30 s after activation was (P < 0.05) lower than the corresponding fresh sperm (82.7 +/- 7.2%). Additionally, the fertilizing capacity of post-thaw sperm was investigated with a standardized sperm to egg ratio 500:1. There is a linear regression relationship between the percentage of motile post-thaw sperm and fertilizing capability. These data demonstrate that 12-21% DMSO can provide good protection to the sperm during the freezing-thawing process. (c) 2006 Elsevier B.V. All rights reserved.; In the present study, the quality of post-thaw sperm of red seabream Pagrus major frozen with 6-24% DMSO was investigated. The motility, average path velocity and fertilizing capacity of fresh and their corresponding post-thaw sperm were examined for evaluation of the post-thaw sperm motion characteristics and its association with fertilizing capacity. An analysis of sperm motility before and after cryopreservation has been performed using computer-assisted sperm analysis (CASA). For post-thaw sperm frozen with 12-21% DMSO, the percentages of motile sperm were not significantly (P > 0.05) changed 10 s after activation. Moreover, the main motility pattern and swimming velocity of the motile post-thaw sperm were not significantly (P > 0.05) changed and the progressive linear motion was still the dominant pattern. However, the total motility of post-thaw sperm (72.3 +/- 6.3%) 30 s after activation was (P < 0.05) lower than the corresponding fresh sperm (82.7 +/- 7.2%). Additionally, the fertilizing capacity of post-thaw sperm was investigated with a standardized sperm to egg ratio 500:1. There is a linear regression relationship between the percentage of motile post-thaw sperm and fertilizing capability. These data demonstrate that 12-21% DMSO can provide good protection to the sperm during the freezing-thawing process. (c) 2006 Elsevier B.V. All rights reserved. |
| 学科主题 | Fisheries ; Marine & Freshwater Biology |
| URL标识 | 查看原文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000244547600003 |
| 公开日期 | 2010-12-24 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/6071]  |
| 专题 | 海洋研究所_海洋生物技术研发中心 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Ctr Marine Biotechnol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100031, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Qing Hua,Li, Jun,Xiao, Zhi Zhong,et al. Use of computer-assisted sperm analysis (CASA) to evaluate the quality of cryopreserved sperm in red seabream (Pagrus major)[J]. AQUACULTURE,2007,263(1-4):20-25. |
| APA | Liu, Qing Hua,Li, Jun,Xiao, Zhi Zhong,Ding, Fu Hong,Yu, Dao De,&Xu, Xi Zhang.(2007).Use of computer-assisted sperm analysis (CASA) to evaluate the quality of cryopreserved sperm in red seabream (Pagrus major).AQUACULTURE,263(1-4),20-25. |
| MLA | Liu, Qing Hua,et al."Use of computer-assisted sperm analysis (CASA) to evaluate the quality of cryopreserved sperm in red seabream (Pagrus major)".AQUACULTURE 263.1-4(2007):20-25. |
入库方式： OAI收割
来源：海洋研究所
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