An efficient methodology for cryopreservation of spermatozoa of red seabream, Pagrus major, with 2-mL cryovials
文献类型：期刊论文
| 作者 | Liu, Qinghua; Li, Jun; Zhang, Shicui; Ding, Fuhong; Xu, Xizhang; Xiao, Zhizhong; Xu, Shihong |
| 刊名 | JOURNAL OF THE WORLD AQUACULTURE SOCIETY
 |
| 出版日期 | 2006-09-01 |
| 卷号 | 37期号:3页码:289-297 |
| 关键词 | Turbot Scophthalmus-maximus Large-scale Fertilization Cryogenic Preservation Sperm Cryopreservation Clarias-gariepinus African Catfish Cryoprotectants Carp Motility Straws |
| ISSN号 | 0893-8849 |
| 文献子类 | Article |
| 英文摘要 | The aim of this study was to optimize the cryopreservation protocols for the sperm of red seabream, Pagrus major. The 2-mL cryovials and programmable freezer were employed for cryopreservation. Six extenders, six cryoprotectants in various concentrations ranging from 6 to 20% (v/v), four cooling rates, and three thawing temperatures were evaluated by postthaw sperm motility and fertility. The ratio of sperm to egg for postthaw sperm fertilization trials was experimentally standardized and was optimal at 500:1. The best motility of postthaw sperm (79.4 +/- 4.7% to 88.6 +/- 8.0%), fertilization rates (89.6 +/- 2.9 to 95.6 +/- 1.9%), and hatching rates (85.3 +/- 5.1% to 91.4 +/- 4.3%) were achieved when Cortland extender, dimethyl sulfoxide (15, 18, and 20%) or ethylene glycol (9, 12%) as cryoprotectants, 20 C/min as the cooling rate, and 40 C as the thawing temperature were employed. Moreover, the results on embryonic development were not significantly different between cryopreserved sperm and fresh sperm during incubation process. In conclusion, these methods of cryopreservation of red seabream sperm are suitable for routine aquaculture application and preservation of genetic resources.; The aim of this study was to optimize the cryopreservation protocols for the sperm of red seabream, Pagrus major. The 2-mL cryovials and programmable freezer were employed for cryopreservation. Six extenders, six cryoprotectants in various concentrations ranging from 6 to 20% (v/v), four cooling rates, and three thawing temperatures were evaluated by postthaw sperm motility and fertility. The ratio of sperm to egg for postthaw sperm fertilization trials was experimentally standardized and was optimal at 500:1. The best motility of postthaw sperm (79.4 +/- 4.7% to 88.6 +/- 8.0%), fertilization rates (89.6 +/- 2.9 to 95.6 +/- 1.9%), and hatching rates (85.3 +/- 5.1% to 91.4 +/- 4.3%) were achieved when Cortland extender, dimethyl sulfoxide (15, 18, and 20%) or ethylene glycol (9, 12%) as cryoprotectants, 20 C/min as the cooling rate, and 40 C as the thawing temperature were employed. Moreover, the results on embryonic development were not significantly different between cryopreserved sperm and fresh sperm during incubation process. In conclusion, these methods of cryopreservation of red seabream sperm are suitable for routine aquaculture application and preservation of genetic resources. |
| 学科主题 | Fisheries |
| 语种 | 英语 |
| WOS记录号 | WOS:000239617000006 |
| 公开日期 | 2010-12-24 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/6149]  |
| 专题 | 海洋研究所_海洋生物技术研发中心 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Ctr Biotechnol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100031, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Ctr Biotechnol R&D, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Qinghua,Li, Jun,Zhang, Shicui,et al. An efficient methodology for cryopreservation of spermatozoa of red seabream, Pagrus major, with 2-mL cryovials[J]. JOURNAL OF THE WORLD AQUACULTURE SOCIETY,2006,37(3):289-297. |
| APA | Liu, Qinghua.,Li, Jun.,Zhang, Shicui.,Ding, Fuhong.,Xu, Xizhang.,...&Xu, Shihong.(2006).An efficient methodology for cryopreservation of spermatozoa of red seabream, Pagrus major, with 2-mL cryovials.JOURNAL OF THE WORLD AQUACULTURE SOCIETY,37(3),289-297. |
| MLA | Liu, Qinghua,et al."An efficient methodology for cryopreservation of spermatozoa of red seabream, Pagrus major, with 2-mL cryovials".JOURNAL OF THE WORLD AQUACULTURE SOCIETY 37.3(2006):289-297. |
入库方式： OAI收割
来源：海洋研究所
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