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PCR-DGGE analysis of intestinal bacteria and effect of Bacillus spp. on intestinal microbial diversity in kuruma shrimp (Marsupenaeus japonicus)

文献类型：期刊论文

作者Liu Huaide1,2; Liu Mei1; Wang Baojie1,2; Jiang Keyong1; Jiang Shan1,2; Sun Shujuan1,2; Wang Lei1
刊名CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
出版日期2010-07-01
卷号28期号:4页码:808-814
ISSN号0254-4059
关键词Intestinal Microbial Diversity Pcr-dgge Kuruma Shrimp
DOI10.1007/s00343-010-9101-7
文献子类Article
英文摘要In this study, the intestinal microbiota of kuruma shrimp (Marsupenaeus japonicus) was examined by molecular analysis of the 16S rDNA to identify the dominant intestinal bacteria and to investigate the effects of Bacillus spp. on intestinal microbial diversity. Samples of the intestines of kuruma shrimp fed normal feed and Bacillus spp. amended feed. PCR and denaturing gradient gel electrophoresis (DGGE) analyses were then performed on DNA extracted directly from the guts. Population fingerprints of the predominant organisms were generated by DGGE analysis of the universal V3 16S rDNA amplicons, and distinct bands in the gels were sequenced. The results suggested that the gut of kuruma shrimp was dominated by Vibrio sp. and uncultured gamma proteobacterium. Overall, the results of this study suggest that PCR-DGGE is a possible method of studying the intestinal microbial diversity of shrimp.; In this study, the intestinal microbiota of kuruma shrimp (Marsupenaeus japonicus) was examined by molecular analysis of the 16S rDNA to identify the dominant intestinal bacteria and to investigate the effects of Bacillus spp. on intestinal microbial diversity. Samples of the intestines of kuruma shrimp fed normal feed and Bacillus spp. amended feed. PCR and denaturing gradient gel electrophoresis (DGGE) analyses were then performed on DNA extracted directly from the guts. Population fingerprints of the predominant organisms were generated by DGGE analysis of the universal V3 16S rDNA amplicons, and distinct bands in the gels were sequenced. The results suggested that the gut of kuruma shrimp was dominated by Vibrio sp. and uncultured gamma proteobacterium. Overall, the results of this study suggest that PCR-DGGE is a possible method of studying the intestinal microbial diversity of shrimp.
学科主题Limnology ; Oceanography
URL标识查看原文
语种英语
WOS记录号WOS:000279294400014
公开日期2010-12-24
源URL[http://ir.qdio.ac.cn/handle/337002/6155]  
专题海洋研究所_海洋生物技术研发中心
作者单位1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
推荐引用方式
GB/T 7714		 Liu Huaide,Liu Mei,Wang Baojie,et al. PCR-DGGE analysis of intestinal bacteria and effect of Bacillus spp. on intestinal microbial diversity in kuruma shrimp (Marsupenaeus japonicus)[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2010,28(4):808-814.
APA Liu Huaide.,Liu Mei.,Wang Baojie.,Jiang Keyong.,Jiang Shan.,...&Wang Lei.(2010).PCR-DGGE analysis of intestinal bacteria and effect of Bacillus spp. on intestinal microbial diversity in kuruma shrimp (Marsupenaeus japonicus).CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,28(4),808-814.
MLA Liu Huaide,et al."PCR-DGGE analysis of intestinal bacteria and effect of Bacillus spp. on intestinal microbial diversity in kuruma shrimp (Marsupenaeus japonicus)".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 28.4(2010):808-814.

入库方式： OAI收割

来源：海洋研究所

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