Identification of Crassostrea ariakensis and related oysters by multiplex species-specific PCR
文献类型：期刊论文
| 作者 | Wang, Haiyan1,2; Guo, Ximing1 |
| 刊名 | JOURNAL OF SHELLFISH RESEARCH
 |
| 出版日期 | 2008-05-01 |
| 卷号 | 27期号:3页码:481-487 |
| 关键词 | Oyster Identification Species-specific Pcr Cytochrome Oxidase i 28s Rrna Ostreidae Suminoe Oyster Crassostrea Ariakensis |
| ISSN号 | 0730-8000 |
| 文献子类 | Article |
| 英文摘要 | Genetic markers are needed for rapid and reliable identification of oysters. In this study, we developed multiplex genus- and species-specific PCR markers for the identification of oysters from China. We used the mitochondrial cytochrome oxidase I (COI) and nuclear 28S ribosomal RNA genes for marker development. DNA sequences from different species were obtained from GenBank or by direct sequencing. Sequences were aligned, and genus- and species-specific nucleotides were identified. Primers were designed for genus/species-specific amplification to generate fragments of different sizes. A multiplex set of genus- and species-specific primers from the 28S gene was able to separate C. ariakensis and C. hongkongensis from other species and assign oysters to four genera. A set of species-specific COI primers provided positive identification of all five Crassostrea species from China, C. ariakensis, C. hongkongensis, C. angulata, C. gigas, and C. sikamea in a single PCR. The multiplex PCR assays do not require fluorescence-labeling or post-PCR enzyme digestion, providing a simple, fast and reliable method for the identification of oysters from China.; Genetic markers are needed for rapid and reliable identification of oysters. In this study, we developed multiplex genus- and species-specific PCR markers for the identification of oysters from China. We used the mitochondrial cytochrome oxidase I (COI) and nuclear 28S ribosomal RNA genes for marker development. DNA sequences from different species were obtained from GenBank or by direct sequencing. Sequences were aligned, and genus- and species-specific nucleotides were identified. Primers were designed for genus/species-specific amplification to generate fragments of different sizes. A multiplex set of genus- and species-specific primers from the 28S gene was able to separate C. ariakensis and C. hongkongensis from other species and assign oysters to four genera. A set of species-specific COI primers provided positive identification of all five Crassostrea species from China, C. ariakensis, C. hongkongensis, C. angulata, C. gigas, and C. sikamea in a single PCR. The multiplex PCR assays do not require fluorescence-labeling or post-PCR enzyme digestion, providing a simple, fast and reliable method for the identification of oysters from China. |
| 语种 | 英语 |
| WOS记录号 | WOS:000256117200001 |
| 公开日期 | 2010-12-30 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/6331]  |
| 专题 | 海洋研究所_海洋生物技术研发中心 |
| 作者单位 | 1.Rutgers State Univ, Inst Marine & Coastal Sci, Haskin Shellfish Res Lab, Piscataway, NJ 08349 USA 2.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Shandong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Haiyan,Guo, Ximing. Identification of Crassostrea ariakensis and related oysters by multiplex species-specific PCR[J]. JOURNAL OF SHELLFISH RESEARCH,2008,27(3):481-487. |
| APA | Wang, Haiyan,&Guo, Ximing.(2008).Identification of Crassostrea ariakensis and related oysters by multiplex species-specific PCR.JOURNAL OF SHELLFISH RESEARCH,27(3),481-487. |
| MLA | Wang, Haiyan,et al."Identification of Crassostrea ariakensis and related oysters by multiplex species-specific PCR".JOURNAL OF SHELLFISH RESEARCH 27.3(2008):481-487. |
入库方式： OAI收割
来源：海洋研究所
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