Its length polymorphism in oysters and its use in species identification
文献类型：期刊论文
| 作者 | Wang, Yongping1,2; Guo, Ximing1 |
| 刊名 | JOURNAL OF SHELLFISH RESEARCH
 |
| 出版日期 | 2008-05-01 |
| 卷号 | 27期号:3页码:489-493 |
| 关键词 | Species Identification Ribosomal Rna Its Genetic Marker Oysters Ostreidae Suminoe Oyster Crassostrea Ariakensis |
| ISSN号 | 0730-8000 |
| 文献子类 | Article |
| 英文摘要 | In an effort to develop genetic markers for oyster identification, we studied length polymorphism in internal transcribed spacers (ITS) between major ribosomal RNA genes in 12 common species of Ostreidae: Crassostrea virginica, C. rhizophorae, C. gigas, C. angulata, C. sikamea, C. ariakensis, C. hongkongensis, Saccostrea echinata, S. glomerata, Ostrea angasi, O. edulis, and O. conchaphila. We designed two pairs of primers and optimized PCR conditions for simultaneous amplification of ITS 1 and ITS2 in a single PCR. Amplification was successful in all 12 species, and PCR products were visualized on high-resolution agarose gels. ITS2 was longer than ITS 1 in all Crassostrea and Saccostrea species, whereas they were about the same size in the three Ostrea species. No intraspecific variation in ITS length was detected. Among species, the length of ITS I and ITS2 was polymorphic and provided unique identification of 8 species or species pairs: C. ariakensis, C. hongkongensis, C. sikamea, O. conchaphila, C. virginica/C. rhizophorae, C. gigas/C. angulata, S. echinata/S. glonzerata, and O. angasi/O. edulis. The ITS assay provides simple, rapid and effective identification of C. ariakensis and several other oyster species. Because the primer sequences are conserved, the ITS assay may be useful in the identification of other bivalve species.; In an effort to develop genetic markers for oyster identification, we studied length polymorphism in internal transcribed spacers (ITS) between major ribosomal RNA genes in 12 common species of Ostreidae: Crassostrea virginica, C. rhizophorae, C. gigas, C. angulata, C. sikamea, C. ariakensis, C. hongkongensis, Saccostrea echinata, S. glomerata, Ostrea angasi, O. edulis, and O. conchaphila. We designed two pairs of primers and optimized PCR conditions for simultaneous amplification of ITS 1 and ITS2 in a single PCR. Amplification was successful in all 12 species, and PCR products were visualized on high-resolution agarose gels. ITS2 was longer than ITS 1 in all Crassostrea and Saccostrea species, whereas they were about the same size in the three Ostrea species. No intraspecific variation in ITS length was detected. Among species, the length of ITS I and ITS2 was polymorphic and provided unique identification of 8 species or species pairs: C. ariakensis, C. hongkongensis, C. sikamea, O. conchaphila, C. virginica/C. rhizophorae, C. gigas/C. angulata, S. echinata/S. glonzerata, and O. angasi/O. edulis. The ITS assay provides simple, rapid and effective identification of C. ariakensis and several other oyster species. Because the primer sequences are conserved, the ITS assay may be useful in the identification of other bivalve species. |
| 语种 | 英语 |
| WOS记录号 | WOS:000256117200002 |
| 公开日期 | 2010-12-30 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/6333]  |
| 专题 | 海洋研究所_海洋生物技术研发中心 |
| 作者单位 | 1.Rutgers State Univ, Inst Marine & Coastal Sci, Haskin Shellfish Res Lab, Port Norris, NJ 08349 USA 2.Chinese Acad Sci, Inst Oceanol, Expt Marine Biol Lab, Qingdao 266071, Shandong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Yongping,Guo, Ximing. Its length polymorphism in oysters and its use in species identification[J]. JOURNAL OF SHELLFISH RESEARCH,2008,27(3):489-493. |
| APA | Wang, Yongping,&Guo, Ximing.(2008).Its length polymorphism in oysters and its use in species identification.JOURNAL OF SHELLFISH RESEARCH,27(3),489-493. |
| MLA | Wang, Yongping,et al."Its length polymorphism in oysters and its use in species identification".JOURNAL OF SHELLFISH RESEARCH 27.3(2008):489-493. |
入库方式： OAI收割
来源：海洋研究所
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