Direct immobilisation of antibodies on a bioinspired architecture as a sensing platform
文献类型：期刊论文
| 作者 | Wan, Yi1,2; Zhang, Dun1; Wang, Yi1 ; Qi, Peng1,2; Hou, Baorong1; Zhang, D (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Corros Sci, 7 Nanhai Rd, Qingdao 266071, Shandong, Peoples R China
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| 刊名 | BIOSENSORS & BIOELECTRONICS
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| 出版日期 | 2011-01-15 |
| 卷号 | 26期号:5页码:2595-2600 |
| ISSN号 | 0956-5663 |
| 关键词 | Dopamine Impedimetric Immunosensor Sensing Platform Bioinspired Architecture Bacteria Detection |
| DOI | 10.1016/j.bios.2010.11.013 |
| 文献子类 | Article |
| 英文摘要 | A sensitive and selective immunosensor for the nonlabeled detection of sulfate-reducing bacteria (SRB) is constructed using a self-polymerised polydopamine film as the immobilisation platform. Self-polymerisation of dopamine is used as a powerful approach for applying multifunctional coatings onto the surface of a gold electrode. The polydopamine film is used not only as the immobilisation platform, but also as a cross-linker reagent for the immobilisation of the anti-SRB antibody. The polydopamine film is loaded with a high density of anti-SRB antibodies linked to the substrate to obtain high response signals. The formation and fabrication of the biosensor and the quantification of antibody anchoring are monitored, and SRB detection is performed by either quartz crystal microbalance (QCM) or electrochemical impedance spectroscopy (EIS). After modeling the impedance Nyquist plots of the SRB/anti-SRB/polydopamine/gold electrode for increasing concentrations of SRB, the electron transfer resistance (R(ct)) is used as a measure of immunocomplex binding. The R(ct) is correlated with the concentration of bacterial cells in the range of 1.8 x 10(2) to 1.8 x 10(6) CFU mL(-1); the detection limit is 50 CFU mL(-1). This work demonstrates a new immobilisation platform for the development of a sensitive and label-less impedimetric and piezoelectric immunosensor. This immunosensor may be broadly applied in clinical diagnoses and the monitoring of water environmental pollution. The method proposed is distinct in its ease of application, use of a simple protocol, and mild reaction conditions. These allow it to be applied to a wide variety of materials. (C) 2010 Elsevier B.V. All rights reserved.; A sensitive and selective immunosensor for the nonlabeled detection of sulfate-reducing bacteria (SRB) is constructed using a self-polymerised polydopamine film as the immobilisation platform. Self-polymerisation of dopamine is used as a powerful approach for applying multifunctional coatings onto the surface of a gold electrode. The polydopamine film is used not only as the immobilisation platform, but also as a cross-linker reagent for the immobilisation of the anti-SRB antibody. The polydopamine film is loaded with a high density of anti-SRB antibodies linked to the substrate to obtain high response signals. The formation and fabrication of the biosensor and the quantification of antibody anchoring are monitored, and SRB detection is performed by either quartz crystal microbalance (QCM) or electrochemical impedance spectroscopy (EIS). After modeling the impedance Nyquist plots of the SRB/anti-SRB/polydopamine/gold electrode for increasing concentrations of SRB, the electron transfer resistance (R(ct)) is used as a measure of immunocomplex binding. The R(ct) is correlated with the concentration of bacterial cells in the range of 1.8 x 10(2) to 1.8 x 10(6) CFU mL(-1); the detection limit is 50 CFU mL(-1). This work demonstrates a new immobilisation platform for the development of a sensitive and label-less impedimetric and piezoelectric immunosensor. This immunosensor may be broadly applied in clinical diagnoses and the monitoring of water environmental pollution. The method proposed is distinct in its ease of application, use of a simple protocol, and mild reaction conditions. These allow it to be applied to a wide variety of materials. (C) 2010 Elsevier B.V. All rights reserved. |
| 学科主题 | Biophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics |
| URL标识 | 查看原文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000286904400130 |
| 公开日期 | 2012-07-03 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/11438]  |
| 专题 | 海洋研究所_海洋腐蚀与防护研究发展中心 |
| 通讯作者 | Zhang, D (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Corros Sci, 7 Nanhai Rd, Qingdao 266071, Shandong, Peoples R China |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Corros Sci, Qingdao 266071, Shandong, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wan, Yi,Zhang, Dun,Wang, Yi,et al. Direct immobilisation of antibodies on a bioinspired architecture as a sensing platform[J]. BIOSENSORS & BIOELECTRONICS,2011,26(5):2595-2600. |
| APA | Wan, Yi,Zhang, Dun,Wang, Yi,Qi, Peng,Hou, Baorong,&Zhang, D .(2011).Direct immobilisation of antibodies on a bioinspired architecture as a sensing platform.BIOSENSORS & BIOELECTRONICS,26(5),2595-2600. |
| MLA | Wan, Yi,et al."Direct immobilisation of antibodies on a bioinspired architecture as a sensing platform".BIOSENSORS & BIOELECTRONICS 26.5(2011):2595-2600. |
入库方式： OAI收割
来源：海洋研究所
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