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Impedimetric immunosensor doped with reduced graphene sheets fabricated by controllable electrodeposition for the non-labelled detection of bacteria

文献类型：期刊论文

作者Wan, Yi1,2; Lin, Zhifeng1,2; Zhang, Dun1; Wang, Yi1; Hou, Baorong1; Zhang, D (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Corros Sci, 7 Nanhai Rd, Qingdao 266071, Shandong, Peoples R China
刊名BIOSENSORS & BIOELECTRONICS
出版日期2011-01-15
卷号26期号:5页码:1959-1964
ISSN号0956-5663
关键词Sulphate-reducing Bacteria Reduced Graphene Sheets Electrochemical Impedance Spectroscopy Chitosan Electrodeposition
DOI10.1016/j.bios.2010.08.008
文献子类Article
英文摘要A facile, sensitive and reliable impedimetric immunosensor doped with reduced graphene sheets (RGSs) and combined with a controllable electrodeposition technique was developed for the selective detection of marine pathogenic sulphate-reducing bacteria (SRB). The morphology of RGSs and the electrochemical properties of RGSs-doped chitosan (CS) nanocomposite film were investigated by atomic force microscopy. Fourier transform infrared spectroscopy, and cyclic voltammetry (CV). Electrochemical impedance spectroscopy and CV were used to verify the stepwise assembly of the sensor system. Faradic impedance spectroscopy for charge transfer for the redox probe Fe(CN)(6)(3-/4-) was done to determine SRB concentrations. The diameter of the Nyquist diagram that is equal to the charge-transfer resistance (R(ct)) increased with increasing SRB concentration. A linear relationship between Rct and SRB concentration was obtained in the SRB concentration range of 1.8 x 10(1) to 1.8 x 10(7) cfu/ml. The impedimetric biosensor gave a distinct response to SRB, but had no obvious response to Vibrio angillarum. It showed a high selectivity for the detection of the pathogen. Based on a combination of the biocompatibility of CS and good electrical conductivity of RGSs, a nanocomposite film with novel architecture was used to immobilize biological and chemical targets and to develop a new type of biosensor. (C) 2010 Elsevier B.V. All rights reserved.; A facile, sensitive and reliable impedimetric immunosensor doped with reduced graphene sheets (RGSs) and combined with a controllable electrodeposition technique was developed for the selective detection of marine pathogenic sulphate-reducing bacteria (SRB). The morphology of RGSs and the electrochemical properties of RGSs-doped chitosan (CS) nanocomposite film were investigated by atomic force microscopy. Fourier transform infrared spectroscopy, and cyclic voltammetry (CV). Electrochemical impedance spectroscopy and CV were used to verify the stepwise assembly of the sensor system. Faradic impedance spectroscopy for charge transfer for the redox probe Fe(CN)(6)(3-/4-) was done to determine SRB concentrations. The diameter of the Nyquist diagram that is equal to the charge-transfer resistance (R(ct)) increased with increasing SRB concentration. A linear relationship between Rct and SRB concentration was obtained in the SRB concentration range of 1.8 x 10(1) to 1.8 x 10(7) cfu/ml. The impedimetric biosensor gave a distinct response to SRB, but had no obvious response to Vibrio angillarum. It showed a high selectivity for the detection of the pathogen. Based on a combination of the biocompatibility of CS and good electrical conductivity of RGSs, a nanocomposite film with novel architecture was used to immobilize biological and chemical targets and to develop a new type of biosensor. (C) 2010 Elsevier B.V. All rights reserved.
学科主题Biophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics
URL标识查看原文
语种英语
WOS记录号WOS:000286904400027
公开日期2012-07-03
源URL[http://ir.qdio.ac.cn/handle/337002/11452]  
专题海洋研究所_海洋腐蚀与防护研究发展中心
通讯作者Zhang, D (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Corros Sci, 7 Nanhai Rd, Qingdao 266071, Shandong, Peoples R China
作者单位1.Chinese Acad Sci, Inst Oceanol, Key Lab Corros Sci, Qingdao 266071, Shandong, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
推荐引用方式
GB/T 7714		 Wan, Yi,Lin, Zhifeng,Zhang, Dun,et al. Impedimetric immunosensor doped with reduced graphene sheets fabricated by controllable electrodeposition for the non-labelled detection of bacteria[J]. BIOSENSORS & BIOELECTRONICS,2011,26(5):1959-1964.
APA Wan, Yi,Lin, Zhifeng,Zhang, Dun,Wang, Yi,Hou, Baorong,&Zhang, D .(2011).Impedimetric immunosensor doped with reduced graphene sheets fabricated by controllable electrodeposition for the non-labelled detection of bacteria.BIOSENSORS & BIOELECTRONICS,26(5),1959-1964.
MLA Wan, Yi,et al."Impedimetric immunosensor doped with reduced graphene sheets fabricated by controllable electrodeposition for the non-labelled detection of bacteria".BIOSENSORS & BIOELECTRONICS 26.5(2011):1959-1964.

入库方式： OAI收割

来源：海洋研究所

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